Foreword
Introduction
1 Scope
2 Normative reference(s)
3 Principle
4 Reagents and Materials
4.1 1-(2-Methoxyphenyl) piperazine (MP)
4.2 9-(Methylaminomethyl) anthracene (MAMA)
4.3 Acetic acid
4.4 Acetic anhydride
4.5 Triethylamine
4.6 Phosphoric acid
4.7 Reagent solvents
4.8 Buffer solution and HPLC mobile phase
4.8.1 Triethylamine buffer - vapor analysis
4.8.2 Sodium acetate buffer-aerosols analysis
4.8.3 Mobile phase - vapor analysis
4.8.4 Mobile phase - aerosol analysis
4.9 Reagent solutions
4.9.1 Desorption solution - vapor analysis
4.9.2 Derivatization solution - aerosols analysis
5 Apparatus
5.1 Sampling system
5.2 Sampling pump
5.3 Tubing
5.4 Flowmeter
5.5 Glass jar
5.6 Liquid chromatographic system
5.7 Autosampler
5.8 HPLC column
6 Air sampling
6.1 Calibration of pump
6.2 Preparation of sampling equipment
6.3 Preparation of MAMA-impregnated filt
6.4 Collection of samples
6.5 Blanks
7 Procedure
7.1 Safety precautions
7.2 Calibration standard
7.2.1 Preparation of monomer derivatives
7.2.2 Preparation of standard solutions from
the isocyanate monomer-urea derivatives (MAMA)
7.2.3 Stability of isocyanate-ureas in solutions
7.2.4 Preparation of standard solution for the
aerosol isocyanates analysis
8 Samples processing
8.1 Vapor analysis
8.2 Aerosol analysis
9 HPLC Conditions
9.1 HPLC Conditions- vapor isocyanates
9.2 HPLC Conditions- aerosol isocyanates
10 Analysis
10.1 Calibration
10.1.1 Calibration curve
10.2 Quality Control
10.2.1 Vapor isocyanates
10.2.2 Aerosol isocyanates
10.3 Sample Quantification
10.3.1 Calculation of vapor isocyanates
10.3.2 Calculation of aerosol isocyanates
11 Interference
12 Determination of performance characteristics
12.1 Introduction
12.2 Relevant uncertainty contributions and criteria
12.3 Assessment of performance characteristics,
following the detailed approach in Reference
ISO/GUM
12.3.1 Collection efficiency - relative to
particle size distribution
12.3.2 Air Sampling
12.3.3 Analysis
12.3.4 Mass of compound in sample blank
12.3.5 Between-laboratory uncertainty contributions
12.3.6 Combined uncertainty
12.3.7 Expanded uncertainty
12.3.8 Uncertainty from performance criteria
13 References
Annex A (informative) - Performance characteristics
A.1 Uncertainty estimates
A.2 Combined uncertainty
A.3 Expanded uncertainty
Annex B (informative) - Example chromatograms