Part of ISO 9308, covers a miniaturized method for the detection and enumeration of Escherichia (E. coli) in waste and surface water by inoculation in a liquid medium. Applies to all types of waste and surface waters, particularly those rich in suspended matter. Method is not suitable for drinking water and any other type of water for which the guideline is less than 15 counts per 100 ml. Method is not appropriate for enumeration and detection of coliform bacteria other than E. coli.