Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of the test sample
9 Procedure .
10Test report
Annex A (informative) Results of interlaboratory trials — Gradient elution HPLC method Annex B (normative) Checking of the titre of the prepared internal standard solution.
Annex C (normative) Preparation and test of purified sulfatase solution and checking of the desulphation step on ion-exchange columns .
Annex D (informative) HPLC system and column performance criteria qualification
Annex E (informative) Elution in the isocratic mode
Bibliography