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  • VDI 4331 Blatt 2:2013-08

    Current The latest, up-to-date edition.

    Monitoring the effects of genetically modified organisms (GMOs) - Method for extracting nucleic acids from soil for the analysis of microbial communities and detection of transgenic DNA - Quality requirements and applications

    Available format(s):  Hardcopy, PDF

    Language(s):  German - English

    Published date:  01-08-2013

    Publisher:  Verlag des Vereins Deutscher Ingenieure

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    Table of Contents - (Show below) - (Hide below)

    Preliminary note
    Introduction
    1 Scope
    2 Normative references
    3 Terms and definitions
    4 Quality requirements for nucleic
      acid extracts
    5 List of materials
    6 Procedure
    Annex A - Protocol for DNA extraction
              from soil samples
    Annex B - Special modification of a
              Protocol for DNA extraction
              from soils with a higher amount
              of humic material
    Annex C - Protocol for simultaneous
              extraction of DNA and RNA
              from soil samples
    Bibliography

    Abstract - (Show below) - (Hide below)

    Nucleic acids extracted from soil can serve as a starting material for the detection of microorganisms living in soil and for assessing their diversity.

    General Product Information - (Show below) - (Hide below)

    Development Note Supersedes VDI 4331-7. (08/2013)
    Document Type Standard
    Publisher Verlag des Vereins Deutscher Ingenieure
    Status Current
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    VDI 4331 Blatt 1:2014-01 Monitoring the effects of genetically modified organisms - Effects on soil organisms
    VDI 4330 Blatt 5:2015-11 Monitoring the effects of genetically modified organisms (GMOs) - Guidelines for the collection and preparation of plant samples for molecular biological analysis

    Standards Referencing This Book - (Show below) - (Hide below)

    VDI 4331 Blatt 1:2014-01 Monitoring the effects of genetically modified organisms - Effects on soil organisms
    EN ISO 21571:2005/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION (ISO 21571:2005/AMD 1:2013)
    ISO 10381-1:2002 Soil quality Sampling Part 1: Guidance on the design of sampling programmes
    ISO 10381-6:2009 Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory
    ISO 24276:2006 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions
    VDI 4330 Blatt 1:2006-10 Monitoring the ecological effects of genetically modified organisms - Genetically modified plants - Basic principles and strategies
    ISO 21571:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction
    VDI 1000:2017-02 VDI Standardisation Work - Principles and procedures
    DIN EN ISO 21571:2013-08 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION (ISO 21571:2005 + AMD 1:2013)
    VDI 4330 Blatt 7:2006-12 Monitoring the effects of genetically modified organisms (GMO) - Qualitative methods for the detection of genetically engineered nucleic acids in the environment
    EN ISO 24276:2006/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - GENERAL REQUIREMENTS AND DEFINITIONS (ISO 24276:2006/AMD 1:2013)
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