• BS EN 16618:2015

    Current The latest, up-to-date edition.

    Food analysis. Determination of acrylamide in food by liquid chromatography tandem mass spectrometry (LC-ESI-MS/MS)

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  30-04-2015

    Publisher:  British Standards Institution

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    1 Scope
    2 Normative references
    3 Principle
    4 Reagents
    5 Apparatus
    6 Sample preparation
    7 Measurement
    8 Determination of concentrations
    9 Precision
    10 Test report
    Annex A (informative) - Typical chromatograms
    Annex B (informative) - Precision data
    Bibliography

    Abstract - (Show below) - (Hide below)

    Defines a method for the determination of acrylamide in bakery ware such as bread, toasted bread, crisp bread, butter cookies, and biscuits, as well as potato products such as potato chips, potato crisps, and potato pan cake and roasted coffee, by liquid chromatography in combination with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS).

    Scope - (Show below) - (Hide below)

    This European Standard specifies a method for the determination of acrylamide in bakery ware such as bread, toasted bread, crisp bread, butter cookies, and biscuits, as well as potato products such as potato chips, potato crisps, and potato pan cake and roasted coffee, by liquid chromatography in combination with electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS). This method has been validated in an interlaboratory study via the analysis of both naturally contaminated and spiked samples, ranging from 14,3µg/kg to 9083µg/kg. It was developed at the Swedish National Food Administration and validated in a study organized by the Directorate General Joint Research Centre (DG JRC), Swedish National Food Administration and the Nordic Committee on Food Analysis (NMKL), see [1] and [2]. The limit of quantification (LOQ) depends on the type of instrument used and on the actual performance of the instrument. The majority of the laboratories participating in the validation study were able to determine acrylamide in a butter cookie sample at a level of 14,3µg/kg. Thus, the validation by interlaboratory study showed that LOQ can be expected to be in the range between below 15µg/kg and 30µg/kg.

    General Product Information - (Show below) - (Hide below)

    Committee AW/275
    Development Note Supersedes 13/30272428 DC. (05/2015)
    Document Type Standard
    Publisher British Standards Institution
    Status Current
    Supersedes

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
    EN ISO 1042 : 1999 LABORATORY GLASSWARE - ONE-MARK VOLUMETRIC FLASKS
    ISO 5725-2:1994 Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
    ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
    EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
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