BS EN ISO 14240-2:2011

Introduction
1 Scope
2 Normative references
3 Definition
4 Principle
5 Materials and reagents
6 Apparatus
7 Fumigation and extraction
8 Determination of carbon in the extracts
9 Precision
Annex A (normative) - Measurement of water-holding
        capacity of soil
Annex B (informative) - Estimation of soil microbial
        biomass in soils containing large amounts of
        living roots - Pre-extraction procedure
Annex C (informative) - Results of an interlaboratory
        trial
Annex D (informative) - Bibliography

Describes a method for the estimation of microbial biomass of soils by measurement of total extractable organic biomass material mainly from freshly killed microorganisms.

This part of ISO 14240 specifies a method for the estimation of microbial biomass of soils by measurement of total extractable organic biomass material mainly from freshly killed microorganisms. The method is also applicable to the estimation of microbial nitrogen and microbial ninhydrin-reactive nitrogen in soil, but this part of ISO 14240 describes only the measurement of extractable organic carbon. The fumigation-extraction (FE) method is applicable to aerobic and anaerobic (water-logged, paddy) soils overthe whole range of soil pH. Biomass can be also measured in soils containing actively decomposing substrates and soils supersaturated with potassium sulfate solution. NOTE Chloroform fumigation also affects soil fauna. The contribution of carbon from these organisms is generally small (< 5 %) and can usually be neglected.