BS ISO 22303:2008

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Preparation of extraction solution and standards
   7.1 Preparation of buffer extraction solution
   7.2 Preparation of internal standard
   7.3 Preparation of calibration standards
   7.4 Setting up of the gas chromatograph and detector
   7.5 Tobacco extraction and isolation of TSNAs
   7.6 Extract concentration
8 Data analysis and calculation of results
9 Repeatability and reproducibility
10 Test report
Annex A (informative) - Typical chromatograms and
        calibration curves
Bibliography

Describes the procedure for the determination of the tobacco specific nitrosamines (TSNAs): N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in ground leaf tobacco, manufactured tobacco and tobacco products.

This International Standard specifies the procedure for the determination of the tobacco specific nitrosamines (TSNAs): N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in ground leaf tobacco, manufactured tobacco and tobacco products. The determination is by means of gas chromatography.