DIN ISO 22030:2005-11

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Materials
   5.1 Test plants
   5.2 Soil and soil storage
        5.2.1 General
        5.2.2 Test soil
        5.2.3 Control soil
   5.3 Reference substance
6 Apparatus
7 Methods
   7.1 Experimental design
   7.2 Pre-test
   7.3 Preliminary test (range-finding)
   7.4 Final test
   7.5 Preparation of the pots
   7.6 Preparation of the seeds
   7.7 Growth conditions
   7.8 Start of the test
   7.9 Handling during the test
        7.9.1 Number of plants and thinning out
        7.9.2 Watering
        7.9.3 Rearrangement of test vessels
        7.9.4 Pollination
        7.9.5 Reporting
   7.10 Endpoint measurements
        7.10.1 Seedling emergence
        7.10.2 Harvest at day 14
        7.10.3 Final harvest
   7.11 Summary and timetable of the test
8 Validity criteria
9 Assessment of the results
   9.1 Presentation of measured data
   9.2 Statistical analysis
        9.2.1 Preliminary test
        9.2.2 Final test
        9.2.3 NOEC (no observed effect concentration) approach
        9.2.4 EC[x] (effect concentration) approach
10 Test report
Annex A (informative) Testing of chemicals in soil
Bibliography

Defines a method for determining the inhibition of the growth and reproductive capability of higher plants by soils under controlled conditions.