EN ISO 15952:2011

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Test environment
6 Reagents
7 Apparatus
8 Storage and preparation of the samples
9 Procedure
10 Reference substance
11 Calculations and expression of results
12 Validity of test for Helix aspersa aspersa
13 Test report
Annex A (normative) - Static method
Annex B (informative) - Breeding technique for snails
Annex C (informative) - Example of composition of
        snail feed
Annex D (informative) Example of table of data
Annex E (informative) Example of results with Helix
        aspersa aspersa
Annex F (informative) Determination of the effects on
        growth by food contamination
Annex G (informative) Test performance with other
        snail species
Bibliography

ISO 15952:2005 specifies a semi-static method for the determination of the effects of contaminants on growth and survival of young snails, usually Helix aspersa aspersa Müller. The animals are exposed via the cutaneous and digestive route using a test substrate (artificial or natural soil according to the objective of the study) to which defined amounts of the following are added: substances or preparations; and soils (contaminated or of unknown quality) or waste materials. A static method is also described.ISO 15952:2005 does not apply to volatile substances, i.e. substances for which the Henry constant, H, or the air/water partition coefficient is over 1, or for which the vapour pressure is over 0,013 3 Pa at 25 °C.This test takes into account possible changes in the test substance, preparation, soil or waste material because the test mixture is prepared and renewed every 7 days during the 28-day test period.