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  • ISO 12966-2:2017

    Current The latest, up-to-date edition.

    Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids

    Available format(s):  Hardcopy, PDF, PDF 3 Users, PDF 5 Users, PDF 9 Users

    Language(s):  English, French

    Published date:  31-03-2017

    Publisher:  International Organization for Standardization

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    Abstract - (Show below) - (Hide below)

    ISO 12966-2:2017 specifies methods of preparing the methyl esters of fatty acids.

    It includes methods for preparing fatty acid methyl esters from animal and vegetable fats and oils, fatty acids and soaps. To cover different requirements, four methylation methods are specified, namely:

    a) a "rapid" transmethylation procedure under alkaline conditions;

    b) a "general" transmethylation/methylation procedure under sequential alkaline and acid conditions;

    c) a BF3 transmethylation procedure;

    d) an alternative procedure using acid-catalysed transmethylation of glycerides.

    Methyl esters so produced are used in various analytical procedures requiring such derivatives, e.g. gas-liquid chromatography (GLC), thin-layer chromatography (TLC) and infrared spectrometry (IR).

    Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.

    General Product Information - (Show below) - (Hide below)

    Development Note Supersedes ISO 5509. (02/2011) Supersedes ISO/DIS 12966-2. (04/2017)
    Document Type Standard
    Publisher International Organization for Standardization
    Status Current
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    14/30286016 DC : 0 BS EN ISO 12966-4 - ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF METHYL ESTERS OF FATTY ACIDS - PART 4: CAPILLARY GAS CHROMATOGRAPHIC METHOD
    13/30281563 DC : 0 BS EN ISO 12966-1 - OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON GAS CHROMATOGRAPHY
    ISO 8312:2015 Rubber compounding ingredients Stearic acid Definition and test methods
    DIN EN ISO 12966-4:2015-11 ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 4: DETERMINATION BY CAPILLARY GAS CHROMATOGRAPHY (ISO 12966-4:2015)
    DIN EN ISO 12966-4 E : 2015 ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 4: DETERMINATION BY CAPILLARY GAS CHROMATOGRAPHY (ISO 129664:2015)
    DIN EN ISO 12966-1 E : 2015 ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014)
    DIN EN ISO 12966-1:2015-10 ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014)
    DIN EN ISO 3657:2013-12 ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE (ISO 3657:2013)
    ISO 3657:2013 Animal and vegetable fats and oils Determination of saponification value
    UNI EN ISO 3657 : 2013 ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE
    EN ISO 12966-1:2014/AC:2015 ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014)
    BS ISO 8312:2015 Rubber compounding ingredients. Stearic acid. Definition and test methods
    BS EN ISO 12966-1:2014 Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Guidelines on modern gas chromatography of fatty acid methyl esters
    BS EN ISO 12966-4:2015 Animal and vegetable fats and oils. Gas chromatography of fatty acid methyl esters Determination by capillary gas chromatography
    I.S. EN ISO 12966-4:2015 ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 4: DETERMINATION BY CAPILLARY GAS CHROMATOGRAPHY (ISO 12966-4:2015)
    I.S. EN ISO 12966-1:2014 ANIMAL AND VEGETABLE FATS AND OILS - GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS - PART 1: GUIDELINES ON MODERN GAS CHROMATOGRAPHY OF FATTY ACID METHYL ESTERS (ISO 12966-1:2014)
    BS EN ISO 3657:2013 Animal and vegetable fats and oils. Determination of saponification value
    I.S. EN ISO 3657:2013 ANIMAL AND VEGETABLE FATS AND OILS - DETERMINATION OF SAPONIFICATION VALUE (ISO 3657:2013)
    UNE-EN ISO 3657:2013 Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2013)
    EN ISO 3657:2013 Animal and vegetable fats and oils - Determination of saponification value (ISO 3657:2013)
    ISO 12966-4:2015 Animal and vegetable fats and oils Gas chromatography of fatty acid methyl esters Part 4: Determination by capillary gas chromatography
    UNE-EN ISO 12966-1:2015 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 1: Guidelines on modern gas chromatography of fatty acid methyl esters (ISO 12966-1:2014)
    EN ISO 12966-4:2015 Animal and vegetable fats and oils - Gas chromatography of fatty acid methyl esters - Part 4: Determination by capillary gas chromatography (ISO 12966-4:2015)

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 1042:1998 Laboratory glassware One-mark volumetric flasks
    ISO 835:2007 Laboratory glassware — Graduated pipettes
    ISO 4799:1978 Laboratory glassware Condensers
    ISO 8655-2:2002 Piston-operated volumetric apparatus Part 2: Piston pipettes
    ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
    ISO 661:2003 Animal and vegetable fats and oils Preparation of test sample
    ISO 78-2:1999 Chemistry Layouts for standards Part 2: Methods of chemical analysis
    ISO 4800:1998 Laboratory glassware Separating funnels and dropping funnels
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