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  • BS EN 14164:2014

    Current The latest, up-to-date edition.

    Foodstuffs. Determination of vitamin B6 by high performance chromatography

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  30-06-2014

    Publisher:  British Standards Institution

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    1 Scope
    2 Normative references
    3 Principle
    4 Reagents
    5 Apparatus
    6 Procedure
    7 Calculation
    8 Test report
    Annex A (informative) - Example of a chromatogram
    Annex B (informative) - Precision data
    Annex C (informative) - Sample treatment option without
                            acid hydrolysis
    Annex D (informative) - Examples for molar absorption
                            coefficients
    Bibliography

    Abstract - (Show below) - (Hide below)

    Describes a method for the determination of vitamin B[6] in foodstuffs by high performance liquid chromatography (HPLC).

    Scope - (Show below) - (Hide below)

    This European Standard specifies a method for the determination of vitamin B6 in foodstuffs by high performance liquid chromatography (HPLC). Vitamin B6 is the mass fraction of the sum of pyridoxine, pyridoxal, pyridoxamine including their phosphorylated derivatives determined as pyridoxine. The β-glycosylated forms are not taken into account. These can be determined with the method given in EN 14663 [1] by which the different vitamins of vitamin B6 (pyridoxal, pyridoxamine and pyridoxine) are separated and individually quantified. A third European Standard, EN 14166 [2], determines the total vitamin B6 by microbiological assay.

    General Product Information - (Show below) - (Hide below)

    Committee AW/275
    Development Note Supersedes DD ENV 14164 & 07/30163680 DC. (07/2008) Supersedes 12/30258767 DC. (06/2014)
    Document Type Standard
    Publisher British Standards Institution
    Status Current
    Supersedes

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories
    EN 14663:2005 Foodstuffs - Determination of vitamin B6 (including its glycosylated forms) by HPLC
    ISO 5725:1986 Precision of test methods Determination of repeatability and reproducibility for a standard test method by inter-laboratory tests
    ISO 3696:1987 Water for analytical laboratory use — Specification and test methods
    EN ISO 3696:1995 Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)
    EN 14166:2009 Foodstuffs - Determination of vitamin B6 by microbiological assay
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