Foreword
Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus
7 Sampling
8 Preparation of test sample
9 Procedure
9.1 Sterol standard solutions
9.1.1 Calibrating solution for the determination
of cholesterol response factor
9.1.2 Qualitative solution for the determination
of sterol retention time
9.2 Test portion
9.3 Saponification
9.4 Extraction of the unsaponifiable matter
9.5 Thin-layer chromatography (TLC)
9.5.1 Preparation of TLC plates
9.5.2 Preparation of glass plate-developing tank
9.5.3 TLC of unsaponifiable matter
9.6 Preparation of silyl derivatives
9.7 Qualitative analysis
9.8 Quantitative analysis
9.8.1 Calculation of response factor
9.8.2 Determination of the test portion
9.8.3 Calculation and expression of results
10 Precision
10.1 Interlaboratory test
10.2 Repeatability
10.3 Reproducibility
11 Test report
Annex A (informative) Examples of the gas-liquid
chromatographic analysis
Annex B (informative) Results of an interlaboratory test
Bibliography