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  • PD ISO/TS 19006:2016

    Current The latest, up-to-date edition.

    Nanotechnologies. 5-(and 6)-Chloromethyl-2’,7’ Dichlorodihydrofluorescein diacetate (CM-H2DCF- DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  31-08-2016

    Publisher:  British Standards Institution

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    Table of Contents - (Show below) - (Hide below)

    Foreword
    Introduction
    1 Scope
    2 Normative references
    3 Terms and definitions
    4 Symbols and abbreviated terms
    5 Materials
    6 Technical equipment
    7 Nanoparticle sample preparation
    8 Preparations
    9 Evaluation of nanoparticle impact on ROS generation in cells
    10 Data analysis and results
    Annex A (informative) - Alternate cell lines
    Annex B (informative) - Alternate fluorescence characterization
            techniques
    Annex C (informative) - Suspension preparation and characterization
    Annex D (informative) - Example experimental data from RAW 264.7
    Bibliography

    Abstract - (Show below) - (Hide below)

    Defines how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H[2]DCFDA assay.

    Scope - (Show below) - (Hide below)

    This document describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.

    The protocol in this document is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

    General Product Information - (Show below) - (Hide below)

    Committee NTI/1
    Document Type Standard
    Publisher British Standards Institution
    Status Current

    Standards Referencing This Book - (Show below) - (Hide below)

    ISO 29701:2010 Nanotechnologies Endotoxin test on nanomaterial samples for in vitro systems Limulus amebocyte lysate (LAL) test
    ISO/TS 18827:2017 Nanotechnologies — Electron spin resonance (ESR) as a method for measuring reactive oxygen species (ROS) generated by metal oxide nanomaterials
    ISO/TR 13097:2013 Guidelines for the characterization of dispersion stability
    ISO/TS 80004-2:2015 Nanotechnologies — Vocabulary — Part 2: Nano-objects
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