09/30198206 DC : 0

Foreword
Introduction
1 Scope
2 Normative reference(s)
3 Principle
4 Reagents and Materials
   4.1 1-(2-Methoxyphenyl) piperazine (MP)
   4.2 9-(Methylaminomethyl) anthracene (MAMA)
   4.3 Acetic acid
   4.4 Acetic anhydride
   4.5 Triethylamine
   4.6 Phosphoric acid
   4.7 Reagent solvents
   4.8 Buffer solution and HPLC mobile phase
        4.8.1 Triethylamine buffer - vapor analysis
        4.8.2 Sodium acetate buffer-aerosols analysis
        4.8.3 Mobile phase - vapor analysis
        4.8.4 Mobile phase - aerosol analysis
   4.9 Reagent solutions
        4.9.1 Desorption solution - vapor analysis
        4.9.2 Derivatization solution - aerosols analysis
5 Apparatus
   5.1 Sampling system
   5.2 Sampling pump
   5.3 Tubing
   5.4 Flowmeter
   5.5 Glass jar
   5.6 Liquid chromatographic system
   5.7 Autosampler
   5.8 HPLC column
6 Air sampling
   6.1 Calibration of pump
   6.2 Preparation of sampling equipment
   6.3 Preparation of MAMA-impregnated filt
   6.4 Collection of samples
   6.5 Blanks
7 Procedure
   7.1 Safety precautions
   7.2 Calibration standard
        7.2.1 Preparation of monomer derivatives
        7.2.2 Preparation of standard solutions from
               the isocyanate monomer-urea derivatives (MAMA)
        7.2.3 Stability of isocyanate-ureas in solutions
        7.2.4 Preparation of standard solution for the
               aerosol isocyanates analysis
8 Samples processing
   8.1 Vapor analysis
   8.2 Aerosol analysis
9 HPLC Conditions
   9.1 HPLC Conditions- vapor isocyanates
   9.2 HPLC Conditions- aerosol isocyanates
10 Analysis
   10.1 Calibration
        10.1.1 Calibration curve
   10.2 Quality Control
        10.2.1 Vapor isocyanates
        10.2.2 Aerosol isocyanates
   10.3 Sample Quantification
        10.3.1 Calculation of vapor isocyanates
        10.3.2 Calculation of aerosol isocyanates
11 Interference
12 Determination of performance characteristics
   12.1 Introduction
   12.2 Relevant uncertainty contributions and criteria
   12.3 Assessment of performance characteristics,
        following the detailed approach in Reference
        ISO/GUM
        12.3.1 Collection efficiency - relative to
               particle size distribution
        12.3.2 Air Sampling
        12.3.3 Analysis
        12.3.4 Mass of compound in sample blank
        12.3.5 Between-laboratory uncertainty contributions
        12.3.6 Combined uncertainty
        12.3.7 Expanded uncertainty
        12.3.8 Uncertainty from performance criteria
13 References
Annex A (informative) - Performance characteristics
   A.1 Uncertainty estimates
   A.2 Combined uncertainty
   A.3 Expanded uncertainty
Annex B (informative) - Example chromatograms