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AS 1766.3.11-1991

Withdrawn

Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Food microbiology Examination of specific products - Dairy products - Test for penicillin

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Withdrawn date

18-05-2018

Published date

01-01-1991

€24.96
Excluding VAT

1 - AS 1766.3.11-1991 FOOD MICROBIOLOGY - EXAMINATION OF SPECIFIC PRODUCTS - DAIRY PRODUCTS - TEST FOR PENICILLIN
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 PRINCIPLE
1 - 3 REAGENTS AND MATERIALS
1 - 3.1 Inhibitor-free reference sample
1 - 3.2 Penicillinase solution
1 - 3.3 Penicillin G
1 - 3.4 Nutrient agar slopes
1 - 3.5 Plate count agar,
2 - 3.6 Tryptone dextrose yeast extract (TDY) broth (see Appendix A),
2 - 3.7 Test organism*,
2 - 3.8 Ethanol
2 - 3.9 Sodium carbonate
2 - 4 APPARATUS
2 - 4.1 Rimless bacteriological test tubes,
2 - 4.2 Petri dishes
2 - 4.3 Paper discs
2 - 4.4 Pipette
2 - 4.5 Forceps
2 - 4.6 Callipers
2 - 4.7 Incubator
2 - 5 SAMPLES
2 - 5.1 Storage of samples
2 - 5.2 Preparation of test samples
2 - 6 CULTURES OF TEST ORGANISM
2 - 6.1 Maintenance of stock culture
2 - 6.2 Preparation of test culture
3 - 7 PROCEDURE
3 - 7.1 Preparation of seeded plates
3 - 7.2 Preparation of reference curve
3 - 7.3 Testing of test samples
4 - 7.4 Confirmation of penicillin
4 - 8 INTERPRETATION OF RESULTS
4 - 8.1 Inhibitory substances not detected
4 - 8.2 Penicillin present in sample
4 - 8.3 Inhibitory substances other than penicillin
4 - 8.4 Penicillin and other inhibitory substances
4 - 9 REPORT
4 - 10 REFERENCES
5 - APPENDIX A - TRYPTONE DEXTROSE YEAST EXTRACT (TDY) BROTH.

This Standard sets out a disc assay method using Bacillus stearothermophilusfor testing milk and other dairy products for penicillin at or above a level of 0.003 µg/mL(0.005 IU/mL).

Committee
FT-024
DocumentType
Standard
ISBN
0 7262 6475 X
Pages
5
PublisherName
Standards Australia
Status
Withdrawn
Supersedes
UnderRevision

This Standard sets out a disc assay method using Bacillus stearothermophilus for testing milk and other dairy products for penicillin at or above a level of 0.003 µg/mL (0.005 IU/mL).NOTES:1 For further reading on the test see Ref. 1.2 The method may be used as either a qualitative or a quantitative test.3 Other inhibitory substances to which the test organism is sensitive may also be detected non-specifically.4 This Method is intended as the reference method. There is another disc assay method (Ref. 2) and also a tube method (Ref. 3) which may be quicker or more convenient for non-reference purposes e.g. routine quality control.

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