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AS
AS 2300.1.2.1-1991

AS 2300.1.2.1-1991

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Current

The latest, up-to-date edition.

Methods of chemical and physical testing for the dairying industry General methods and principles - Determination of nitrogen - Reference Kjeldahl method (Reconfirmed 2019)

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English

Published date

09-08-1991

Publisher

Standards Australia

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Hardcopy - English
PDF 1 User - English
PDF 3 Users - English
PDF 5 Users - English
PDF 9 Users - English
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1 - AS 2300.1.2.1-1991 METHODS OF CHEMICAL AND PHYSICAL TESTING FOR THE DAIRYING INDUSTRY - GENERAL METHODS AND PRINCIPLES - DETE
1 - PREFACE
1 - FOREWORD
1 - METHOD
1 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 PRINCIPLE
2 - 4 REACTIONS
2 - 5 REAGENTS
2 - 5.1 General
2 - 5.2 Sulfuric acid,
2 - 5.3 Copper sulfate,
2 - 5.4 Potassium sulfate,
2 - 5.5 Hydrogen peroxide,
2 - 5.6 Sodium hydroxide,
2 - 5.7 Boric acid,
2 - 5.8 Sulfuric acid,
2 - 5.9 Mixed indicator
2 - 5.10 Tryptophan (C 11 H 12 N 2 O 2 )
2 - 5.11 Ammonium sulphate
2 - 6 APPARATUS
2 - 6.1 Blender(s),
2 - 6.2 Kjeldahl flasks,
2 - 6.3 Liebig condensers,
2 - 6.4 Splash-heads,
2 - 6.5 Erlenmeyer flasks,
2 - 6.6 Glass beads or anti-bumping granules
2 - 6.7 Heating device,
2 - 6.8 pH meter,
3 - 7 NITROGEN RECOVERY TESTS
3 - 7.1 General
3 - 7.2 Tryptophan recovery test
3 - 7.3 Ammonium sulfate recovery test
3 - 7.4 Corrective action
3 - 8 PREPARATION OF TEST SAMPLE
3 - 8.1 Liquid milks and cream
3 - 8.2 Dried milks, caseinates and coprecipitates
3 - 8.3 Ice cream and frozen milk products
4 - 8.4 Cheese
4 - 8.5 Sweetened condensed milk
4 - 8.6 Evaporated milk
4 - 8.7 Yogurt
4 - 8.8 Caseins
4 - 9 TEST PORTIONS
5 - 10 PROCEDURE
5 - 10.1 Blank test
5 - 10.2 Method
5 - 11 CALCULATION
5 - 12 PRECISION
6 - 12.1 Repeatability
6 - 12.2 Reproducibility
6 - 13 TEST REPORT
6 - 14 REFERENCES
7 - APPENDIX A - MODIFIED KJELDAHL PROCEDURES
7 - A1 SCOPE
7 - A2 BLOCK DIGESTION APPARATUS
7 - A3 STEAM DISTILLATION
7 - A4 REDUCED TEST PORTION

This Standard sets out the reference Kjeldahl method for the determination of nitrogen in milk, milk fractions and milk products. The method allows for the estimation of nitrogen on a mass/mass basis and a mass/volume basis.

Committee
FT/10
DocumentType
Standard
ISBN
0 7262 6997 2
Pages
7
ProductNote
Reconfirmed 15/11/2019.
This standard has been reconfirmed in Australia in 2019 and remains current in New Zealand. Reconfirmation Notice 15/11/2019
PublisherName
Standards Australia
Status
Current
Supersedes

This Standard sets out the reference Kjeldahl method for the determination of nitrogen in milk, milk fractions and milk products. The method allows for the estimation of nitrogen on a mass/mass basis and a mass/volume basis.This method is applicable to test portions containing at least 2 mg of nitrogen. Nitrogen present as nitrate or nitrite is not determined.NOTE: Information is given in Appendix A on the use of modified Kjeldahl systems which have been shown to provide results of similar precision to this reference method. These modifications may be used if validated.

For history before 1988 see Preface.
First published as AS 2300.1.2.1-1988.
Second edition 1991.

AS 2300.1.2.2-1988 Methods of chemical and physical testing for the dairying industry - General methods and principles - Determination of nitrogen - Nitrogen fractions from milk
AS 1152-1993 Specification for test sieves
AS 2300.1.6-1989 Methods of chemical and physical testing for the dairying industry General methods and principles - Determination of pH

AS 2300.1.8.2-1991 Methods of chemical and physical testing for the dairying industry General methods and principles - Assessment of instrumental methods - Infrared spectrometric analysis of milk
AS 2300.5.2-1991 Methods of chemical and physical testing for the dairying industry Condensed milk - Determination of sucrose - Polarimetric method (Reconfirmed 2016)
AS 2300.1.2.2-1988 Methods of chemical and physical testing for the dairying industry - General methods and principles - Determination of nitrogen - Nitrogen fractions from milk
AS 2300.5.1-1991 Methods of chemical and physical testing for the dairying industry Condensed milk - General information and preparation of samples (Reconfirmed 2019)
AS 2300.4.8-1994 Methods of chemical and physical testing for the dairying industry Dried milk and dried milk products - Determination of undenatured whey protein nitrogen (UDWPN) in dried skim milk (Reconfirmed 2019)
AS 2300.1.2.2-2008 Methods of chemical and physical testing for the dairying industry General methods and principles-Determination of nitrogen - Nitrogen fractions from milk (Reconfirmed 2019)
AS 2300.10.1-1991 Methods of chemical and physical testing for the dairying industry Caseins, caseinates and coprecipitates - General information and preparation of samples
AS 2300.4.1-1994 Methods of chemical and physical testing for the dairying industry Dried milk and dried milk products - General information and preparation of samples (Reconfirmed 2019)
AS 2300.1.8-2008 Methods of chemical and physical testing for the dairying industry General methods and principles - Assessment of instrumental methods - Protein in milk and infrared spectrometric analysis of milk (Reconfirmed 2019)
AS 2300.9-1990
AS 2300.1.8.1-1988 Methods of chemical and physical testing for the dairying industry - General methods and principles - Analytical quality assurance of instrumental methods - Protein in milk

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