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AS 2300.4.8-1994

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Current

The latest, up-to-date edition.

Methods of chemical and physical testing for the dairying industry Dried milk and dried milk products - Determination of undenatured whey protein nitrogen (UDWPN) in dried skim milk (Reconfirmed 2019)

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English

Published date

05-12-1994

1 - AS 2300.4.8-1994 METHODS OF CHEMICAL AND PHYSICAL TESTING FOR THE DAIRYING INDUSTRY - DRIED MILK AND DRIED MILK PRODUCTS - DE
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 PRINCIPLE
1 - 4 REAGENTS (See also Appendix A)
1 - 4.1 General
2 - 4.2 Sodium chloride
2 - 4.3 Amido black dye solution
2 - 4.4 Citric acid monohydrate
2 - 4.5 Sodium chloride, saturated solution
2 - 4.6 Standard dried skim milk powder
2 - 5 APPARATUS (See also Appendix A)
2 - 5.1 Balance
2 - 5.2 Stoppered test tubes
2 - 5.3 Filter funnel
2 - 5.4 Filter papers
2 - 5.5 One- mark pipettes
2 - 5.6 Capillary pipette or pipettor
2 - 5.7 One-mark volumetric flasks
2 - 5.8 Water bath
2 - 5.9 Glass centrifuge tubes
2 - 5.10 Centrifuge
2 - 5.11 Spectrophotometer
2 - 6 TEST SAMPLES
3 - 7 PROCEDURE
3 - 7.1 Determination
3 - 7.2 Zero UDWPN check
3 - 7.3 Standard curve
4 - 8 CALCULATION
4 - 9 REPEATABILITY
4 - 10 TEST REPORT
4 - 11 REFERENCES
5 - APPENDIX A - METHOD FOR DETERMINING THE UDWPN CONTENT OF THE STANDARD DRIED SKIM MILK POWDER
5 - A1 SCOPE
5 - A2 PRINCIPLE
5 - A3 REAGENTS
5 - A3.1 General
5 - A3.2 Sodium chloride
5 - A3.3 Hydrochloric acid solution (8. 5 g HCl/ 100 mL)
5 - A3.4 Reagents for the Kjeldahl determination of nitrogen
5 - A4 APPARATUS
5 - A4.1 Erlenmeyer flasks
5 - A4.2 Erlenmeyer flasks
5 - A4.3 Bulb pipettes
5 - A4.4 Straight pipette
5 - A4.5 Filter funnels
5 - A4.6 Watch- glasses
5 - A4.7 Filter papers
5 - A4.8 Water bath
5 - A4.9 Centrifuge bottles
5 - A4.10 Centrifuge
6 - A4.11 Apparatus for the Kjeldahl determination of nitrogen
6 - A4.12 pH meter
6 - A5 TEST SAMPLE
6 - A6 PROCEDURE
6 - A6.1 Determination
6 - A6.2 Blank test
7 - A7 CALCULATION

This Standard sets out a method for the determination of the undenatured whey protein nitrogen (UDWPN) content of dried skim milk.

Committee
FT/10
DocumentType
Standard
ISBN
0 7262 9344 2
Pages
7
ProductNote
Reconfirmed 22/11/2019.
This standard has been reconfirmed in Australia in 2019 and remains current in New Zealand. Reconfirmation Notice 22/11/2019
PublisherName
Standards Australia
Status
Current
Supersedes

This Standard sets out a method for the determination of the undenatured whey protein nitrogen (UDWPN) content of dried skim milk.NOTE: The dye-binding method given is suitable for the testing of a large number of samples. If the number of samples is small, the UDWPN content may be determined directly by the Kjeldahl method described in Appendix A.

First published as part of AS N67-1970.
Revised and redesignated in part as AS 1629-1974.
Revised and redesignated in part as AS 2300.4.8-1994.

AS 2167-1996 Graduated straight pipettes
AS 2300.1.2.1-1991 Methods of chemical and physical testing for the dairying industry General methods and principles - Determination of nitrogen - Reference Kjeldahl method (Reconfirmed 2019)
AS 2300.4.1-1994 Methods of chemical and physical testing for the dairying industry Dried milk and dried milk products - General information and preparation of samples (Reconfirmed 2019)
AS 2167-2005 Graduated straight pipettes
AS 2166-2002 Laboratory glassware - One-mark pipettes
AS 2166-1995 One-mark pipettes
AS 2164-2003 Laboratory glassware - One-mark volumetric flasks
AS 2164-1995 Laboratory glassware - One-mark volumetric flasks
AS 2300.1.6-1989 Methods of chemical and physical testing for the dairying industry General methods and principles - Determination of pH

AS 2300.4.1-1994 Methods of chemical and physical testing for the dairying industry Dried milk and dried milk products - General information and preparation of samples (Reconfirmed 2019)

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