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AS
AS 4276.1-1995

AS 4276.1-1995

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Water microbiology General information and procedures

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Superseded date

26-05-2024

Superseded by

AS/NZS 4276.1:2007

Published date

05-03-1995

Publisher

Standards Australia

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Hardcopy - English
PDF 1 User - English
PDF 3 Users - English
PDF 5 Users - English
PDF 9 Users - English
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1 - AS 4276.1-1995 WATER MICROBIOLOGY - GENERAL INFORMATION AND PROCEDURES
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
2 - 3 TYPES OF WATER
2 - 4 USE OF THE METHODS
2 - 5 GOOD LABORATORY PRACTICE
3 - 6 SAMPLING AND SAMPLES
3 - 6.1 General
3 - 6.2 Storage of samples
3 - 7 GLASSWARE
3 - 8 CLEANING OF NEW GLASSWARE, RUBBERWARE, AND NON-STERILE PLASTICS
3 - 8.1 New borosilicate glass, rubberware, and non-sterile plastics
3 - 8.2 New soda glass
4 - 9 CLEANING OF USED LABORATORY EQUIPMENT
4 - 9.1 Preliminary treatment
4 - 9.2 Use of washing machines
4 - 9.3 Test tubes, Petri dishes, flasks and bottles
4 - 9.4 Pipettes
4 - 9.5 Rubber stoppers and sealing liners
4 - 9.6 Caps
5 - 10 STERILIZATION OF EQUIPMENT
5 - 10.1 General
5 - 10.2 Oven method
5 - 10.3 Autoclave method
5 - 11 MEDIA, DILUENTS AND REAGENTS
6 - 12 REFERENCE CULTURES
6 - 13 INCUBATION TEMPERATURES
6 - 14 DILUTIONS
6 - 14.1 General
6 - 14.2 Preparation of dilutions
6 - 15 GENERAL PROCEDURE FOR ESTIMATING THE MOST PROBABLE NUMBER (MPN) OF MICROORGANISMS
6 - 15.1 Principle
6 - 15.2 Diluents and culture media
6 - 15.3 Apparatus
7 - 15.4 Preparation of dilutions and inoculation of medium
7 - 15.4.1 General
7 - 15.4.2 Procedure
7 - 15.5 Incubation
7 - 15.6 Calculation
9 - 16 GENERAL PROCEDURE FOR COLONY COUNTS USING MEMBRANE FILTRATION
9 - 16.1 Principle
9 - 16.2 Diluents and culture media
9 - 16.3 Apparatus
11 - 16.4 Preparation of apparatus and materials
11 - 16.4.1 Sterilization
11 - 16.4.2 Medium
11 - 16.5 Filtration procedure
11 - 16.5.1 General
11 - 16.5.2 Procedure
12 - 16.6 Incubation
12 - 16.7 Staining, counting and identification
12 - 16.8 Calculation
13 - 17 REFERENCES

This Standard sets out general information on the microbiological examination of water using the methods in this series.

Committee
FT-020
DocumentType
Standard
ISBN
0 7262 9605 8
Pages
13
PublisherName
Standards Australia
Status
Superseded
SupersededBy
Supersedes
UnderRevision

This Standard sets out general information on the microbiological examination of water using the methods in this series.

First published as part of AS 1095.4.1.1-1981.
Revised and redesignated in part as AS 4276.1-1995.

AS 2182-1998 Sterilizers - Steam - Benchtop
AS 2252.2-1994 Biological safety cabinets - Laminar flow biological safety cabinets (Class II) for personnel, environment and product protection
AS 2192-1991 Sterilizers - Steam - Downward displacement
AS 2031.2-1987 Selection of containers and preservation of water samples for chemical and microbiological analysis - Microbiological
AS 2252.1-1994 Biological safety cabinets - Biological safety cabinets (Class I) for personnel and environment protection

AS/NZS 4276.19:2001 Water microbiology Examination for thermophilic spp.- Membrane filtration
AS 4276.11-1995 Water microbiology - Pseudomonads Membrane filtration method
AS 4276.13-1995 Water microbiology Pseudomonas aeruginosa - Membrane filtration method
AS 4276.21-2005 Water microbiology Examination for coliforms and Escherichia coli - Determination of most probable number (MPN) using enzyme hydrolysable substrates
AS/NZS 4276.17.1:2000 Water microbiology Spores of sulfite-reducing anaerobes (clostridia) including filtration method
AS/NZS 4276.3.1:2007 Water microbiology Heterotrophic colony count methods - Pour plate method using yeast extract agar
AS 4276.3.1-1995 Water microbiology Heterotrophic colony count methods - Pour plate method using plate count agar
AS 4276.6-1995 Water microbiology Thermotolerant coliforms and Escherichia coli - Estimation of most probable number (MPN)
AS/NZS 4276.20:2003 Water microbiology Examination for coagulase positive staphylococci, including Staphylococcus aureus, by membrane filtration
AS 4276.4-1995 Water microbiology Coliforms - Estimation of most probable number (MPN)
AS/NZS 4276.18:2001 Water microbiology - Aeromonas by membrane filtration including selected speciation
AS 4276.5-1995 Water microbiology Coliforms - Membrane filtration method
AS 4276.14-1995 Water microbiology Salmonellae
AS/NZS 4276.17.2:2000 Water microbiology Spores of sulfite-reducing anaerobes (clostridia) including - Estimation of most probable number (MPN) using the multiple tube dilution technique
AS 4276.9-1995 Water microbiology Faecal streptococci - Membrane filtration method
AS 4276.7-1995 Water microbiology Thermotolerant coliforms and Escherichia coli - Membrane filtration method
AS/NZS 4276.3.2:2003 Water microbiology Heterotrophic colony count methods - Plate count of water containing biocides (Reconfirmed 2013)
AS 4276.10-1995 Water microbiology Pseudomonads - Estimation of most probable number (MPN)
AS 4276.12-1995 Water microbiology Pseudomonas aeruginosa - Estimation of most probable number (MPN)
AS 4276.8-1995 Water microbiology Faecal streptococci - Estimation of most probable number (MPN)

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