AS 4276.13-1995

1 - AS 4276.13-1995 WATER MICROBIOLOGY - PSEUDOMONAS AERUGINOSA-MEMBRANE FILTRATION METHOD
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA AND REAGENT
1 - 3.1 m-PA-C agar
1 - 3.2 Cetrimide B agar
1 - 3.3 Nutrient agar
1 - 3.4 Milk agar (Brown and Scott Foster modification)
1 - 3.5 Oxidase reagent (Kovacs’)
2 - 4 APPARATUS
2 - 4.1 Apparatus and materials for membrane filtration
2 - 4.2 Incubators or water baths
2 - 4.3 Ultraviolet (UV) lamp
2 - 5 REFERENCE CULTURES
2 - 5.1 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495
2 - 5.2 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803
2 - 5.3 Use of reference cultures
2 - 6 SAMPLES
2 - 6.1 Laboratory samples
2 - 6.2 Dilutions
2 - 7 PROCEDURE
2 - 7.1 Controls
2 - 7.2 Filtration procedure
2 - 7.3 Selective enrichment for presumptive
3 - 7.4 Confirmatory tests
3 - 8 TEST REPORT
3 - 9 BIBLIOGRAPHY
4 - APPENDIX A - FLOW DIAGRAM FOR PSEUDOMONAS AERUGINOSA-MEMBRANE FILTRATION METHOD

AS 4276.13-1995 sets out a method, using membrane filtration, for enumerating Pseudomonas aeruginosa in water.

This Standard sets out a method, using membrane filtration, for enumerating Pseudomonas aeruginosa in water.NOTES:1 Membrane filtration is suitable for enumerating microorganisms only when the turbidity of the water is low.2 Bacterial counts derived from membranes with fewer than 20 or more than 80 target colonies are approximate.3 A flow diagram of the procedure is shown in Appendix A.

First published as AS 1095.4.1.13-1981.
Revised and redesignated AS 4276.13-1995.