AS 4276.14-1995

1 - AS 4276.14-1995 WATER MICROBIOLOGY - SALMONELLAE
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA, DILUENTS AND REAGENTS
1 - 3.1 Buffered peptone water
1 - 3.2 Rappaport-Vassiliadis (RV) medium
1 - 3.3 Mannitol selenite cystine (MSC) broth
1 - 3.4 Xylose lysine desoxycholate (XLD) agar
1 - 3.5 Bismuth sulfite agar
1 - 3.6 Nutrient agar
1 - 3.7 Urea agar
1 - 3.8 Sucrose peptone water
1 - 3.9 Lysine decarboxylase broth and decarboxylase broth base
1 - 3.10 ONPG broth
1 - 3.11 Formalinized saline solution
1 - 3.12 Oxidase reagent (Kovacs’)
2 - 4 APPARATUS
2 - 4.1 Usual bacteriological glassware
2 - 4.2 Apparatus for concentration of microorganisms
2 - 4.3 Incubators or water baths
2 - 5 REFERENCE CULTURES AND ANTISERA
2 - 5.1 Salmonella Salford, 16: 1,v: e,n,x, (IMVS culture No. 1710*)
2 - 5.2 Salmonella IIIb, 61: i: z53 (formerly S. arizonae), (IMVS culture No. 1679*)
2 - 5.3 Citrobacter freundii, NCTC 9750 or ATCC 8090
2 - 5.4 Use of reference cultures
2 - 5.5 Salmonella antisera
2 - 5.5.1 Polyvalent 'O' (sinatuc)
2 - 5.5.2 Polyvalent 'H' specific and non-specific (flagellar)
2 - 5.5.3 'Vi' antiserum
2 - 6 LABORATORY SAMPLES
2 - 7 CONCENTRATION TECHNIQUES
2 - 7.1 Moore swab
2 - 7.2 Membrane filtration
2 - 7.3 Centrifugation
3 - 7.4 Diatomaceous earth filtration
3 - 8 RESUSCITATION (PRE-ENRICHMENT)
3 - 9 SELECTIVE BROTH ENRICHMENT
4 - 10 SELECTIVE AGAR CULTURES
4 - 11 PURITY CHECK AND OXIDASE TEST
4 - 12 BIOCHEMICAL CONFIRMATION
4 - 12.1 General
4 - 12.2 Commercial identification kits
4 - 12.3 Biochemical tests
5 - 13 SEROLOGICAL CONFIRMATION
5 - 13.1 General
5 - 13.2 Pretesting the antisera
5 - 13.3 Confirming test cultures
6 - 14 INTERPRETATION OF RESULTS
6 - 15 FURTHER TESTS
6 - 16 COMPLETE IDENTIFICATION
7 - 17 REPORT
7 - 18 REFERENCES
8 - APPENDIX A - FLOW DIAGRAM FOR SALMONELLAE

This Standard sets out a method for the detection of salmonellae in water.

This Standard sets out a method for the detection of salmonellae in water.NOTE: A flow diagram of the procedure is shown in Appendix A.

First published as AS 1095.4.1.9-1981.
Revised and redesignated AS 4276.14-1995.
Incorporating: Amdt 1-1995