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AS
AS 4276.4-1995

AS 4276.4-1995

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Water microbiology Coliforms - Estimation of most probable number (MPN)

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Superseded date

21-02-2024

Superseded by

AS/NZS 4276.6:2007

Language(s)

English

Published date

01-01-1995

Publisher

Standards Australia

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Hardcopy - English
PDF 1 User - English
PDF 3 Users - English
PDF 5 Users - English
PDF 9 Users - English
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1 - AS 4276.4-1995 WATER MICROBIOLOGY - CALIFORMS-ESTIMATION OF MOST PROBABLE NUMBER (MPN)
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA AND REAGENT
1 - 3.1 Improved formate lactose glutamate (IFLG) medium
1 - 3.2 Eosin methylene blue (EMB) agar
1 - 3.3 Lactose peptone (LP) water
1 - 3.4 Nutrient agar
1 - 3.5 Oxidase reagent (Kovacs’)
1 - 4 APPARATUS
1 - 4.1 Rimless bacteriological test tubes
2 - 4.2 Suitable containers for 50 mL tests
2 - 4.3 Pipettes or equivalent measuring devices
2 - 4.4 Incubators or water baths
2 - 5 REFERENCE CULTURES
2 - 5.1 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 1976
2 - 5.2 Pseudomonas aerugionsa, NCTC 10332 or ATCC 10145 or ACM 495
2 - 5.3 Use of reference cultures
2 - 6 SAMPLES
2 - 6.1 Laboratory samples
2 - 6.2 Dilutions
2 - 7 PROCEDURE
2 - 7.1 Presumptive test
3 - 7.2 Confirmatory tests
3 - 8 ESTIMATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS
4 - 9 TEST REPORT
4 - 10 REFERENCE
5 - APPENDIX A - FLOW DIAGRAM FOR COLIFORMS-MOST PROBABLE NUMBER

AS 4276.4-1995 sets out a method, using a multiple tube dilution technique, for estimating the most probable number (MPN) of coliforms in water.

Committee
FT-020
DocumentType
Standard
ISBN
0 7262 9609 0
Pages
5
PublisherName
Standards Australia
Status
Superseded
SupersededBy
Supersedes
UnderRevision

This Standard sets out a method, using a multiple tube dilution technique, for estimating the most probable number (MPN) of coliforms in water.NOTE: A flow diagram of the procedure is shown in Appendix A.

First published as AS 1095.4.1.3-1981.
Revised and redesignated AS 4276.4-1995.

AS 4276.1-1995 Water microbiology General information and procedures
AS 4276.2-1995 Water microbiology Culture media, diluents and reagents (Reconfirmed 2013)

AS 4276.21-2005 Water microbiology Examination for coliforms and Escherichia coli - Determination of most probable number (MPN) using enzyme hydrolysable substrates

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