AS 4276.4-1995

1 - AS 4276.4-1995 WATER MICROBIOLOGY - CALIFORMS-ESTIMATION OF MOST PROBABLE NUMBER (MPN)
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA AND REAGENT
1 - 3.1 Improved formate lactose glutamate (IFLG) medium
1 - 3.2 Eosin methylene blue (EMB) agar
1 - 3.3 Lactose peptone (LP) water
1 - 3.4 Nutrient agar
1 - 3.5 Oxidase reagent (Kovacs’)
1 - 4 APPARATUS
1 - 4.1 Rimless bacteriological test tubes
2 - 4.2 Suitable containers for 50 mL tests
2 - 4.3 Pipettes or equivalent measuring devices
2 - 4.4 Incubators or water baths
2 - 5 REFERENCE CULTURES
2 - 5.1 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 1976
2 - 5.2 Pseudomonas aerugionsa, NCTC 10332 or ATCC 10145 or ACM 495
2 - 5.3 Use of reference cultures
2 - 6 SAMPLES
2 - 6.1 Laboratory samples
2 - 6.2 Dilutions
2 - 7 PROCEDURE
2 - 7.1 Presumptive test
3 - 7.2 Confirmatory tests
3 - 8 ESTIMATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS
4 - 9 TEST REPORT
4 - 10 REFERENCE
5 - APPENDIX A - FLOW DIAGRAM FOR COLIFORMS-MOST PROBABLE NUMBER

AS 4276.4-1995 sets out a method, using a multiple tube dilution technique, for estimating the most probable number (MPN) of coliforms in water.

This Standard sets out a method, using a multiple tube dilution technique, for estimating the most probable number (MPN) of coliforms in water.NOTE: A flow diagram of the procedure is shown in Appendix A.

First published as AS 1095.4.1.3-1981.
Revised and redesignated AS 4276.4-1995.