AS 4276.5-1995

1 - AS 4276.5-1995 WATER MICROBIOLOGY - COLIFORMS-MEMBRANE FILTRATION METHOD
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA AND REAGENT
1 - 3.1 Membrane lauryl sulfate (MLS) agar
1 - 3.2 Membrane lauryl sulfate (MLS) broth
1 - 3.3 Lactose peptone (LP) water
2 - 3.4 Nutrient agar
2 - 3.5 Oxidase reagent (Kovacs’)
2 - 4 APPARATUS
2 - 4.1 Apparatus and materials for membrane filtration
2 - 4.2 Incubators or water baths
2 - 5 REFERENCE CULTURES
2 - 5.1 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 1976
2 - 5.2 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495
2 - 5.3 Use of reference cultures
2 - 6 SAMPLES
2 - 6.1 Laboratory samples
2 - 6.2 Dilutions
2 - 7 PROCEDURE
2 - 7.1 Preparation of plates or pads
2 - 7.2 Controls
2 - 7.3 Filtration procedure
2 - 7.4 Resuscitation and incubation
2 - 7.5 Identification of presumptive coliforms
3 - 7.6 Confirmatory tests
3 - 8 TEST REPORT
4 - 9 REFERENCE
5 - APPENDIX A - FLOW DIAGRAM FOR COLIFORMS-MEMBRANE FILTRATION METHOD

AS 4276.5-1995 sets out a method, using membrane filtration, for enumerating coliforms in water.

This Standard sets out a method, using membrane filtration, for enumerating coliforms in water.NOTES:1 Membrane filtration is suitable for enumerating microorganisms only when the turbidity of the water is low.2 The membrane filtration method is not suitable for waters containing only small numbers of coliforms in the presence of many other species that are capable of growth on the medium used.3 Bacterial counts derived from membranes with fewer than 20 or more than 80 coliform colonies are approximate.4 A flow diagram of the procedure is shown in Appendix A.

First published as AS 1095.4.1.5-1981.
Revised and redesignated AS 4276.5-1995.