AS 4276.9-1995

1 - AS 4276.9-1995 WATER MICROBIOLOGY - FAECAL STREPTOCOCCI-MEMBRANE FILTRATION METHOD
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENTS
1 - 3 CULTURE MEDIA AND REAGENT
1 - 3.1 M-enterococcus agar
1 - 3.2 Bile aesculin agar
1 - 3.3 Brain heart infusion (BHI) broth
2 - 3.4 Brain heart infusion (BHI) agar
2 - 3.5 Catalase reagent
2 - 4 APPARATUS
2 - 4.1 Apparatus and materials for membrane filtration
2 - 4.2 Incubators or water baths
2 - 5 REFERENCE CULTURES
2 - 5.1 Enterococcus faecalis, NCTC 775 or ATCC 19433 or ACM 2517
2 - 5.2 Staphylococcus aureus, ATCC 25923
2 - 5.3 Use of reference cultures
2 - 6 SAMPLES
2 - 6.1 Laboratory samples
2 - 6.2 Dilutions
2 - 7 PROCEDURE
2 - 7.1 Controls
2 - 7.2 Filtration procedure
2 - 7.3 Presumptive test
3 - 7.4 Confirmatory tests
3 - 8 TEST REPORT
4 - APPENDIX A - FLOW DIAGRAM FOR FAECAL STREPTOCOCCI-MEMBRANE FILTRATION METHOD

AS 4276.9-1995 sets out a method, using membrane filtration, for enumerating faecal streptococci in water.

This Standard sets out a method, using membrane filtration, for enumerating faecal streptococci in water.NOTES:1 This method may not be suitable for strains of Streptococcus bovis and Streptococcus equinus that do not grow at 44C to 45C.2 Membrane filtration is suitable for enumerating microorganisms only when the turbidity of the water is low.3 Bacterial counts derived from membranes with fewer than 20 or more than 80 target colonies are approximate.4 A flow diagram of the procedure is shown in Appendix A.

First published as AS 1095.4.1.8-1981.
Revised and redesignated AS 4276.9-1995.