AS/NZS 1766.2.12:2002

1 - AS/NZS 1766.2.12:2002 FOOD MICROBIOLOGY - EXAMINATION FOR SPECIFIC ORGANISMS-ESCHERICHIA COLI IN BIVALVE MOLLUSCS-RAPID...
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 DEFINITION
2 - 3.1 Escherichia coli
2 - 4 CULTURE MEDIA, DILUENTS AND REAGENTS
2 - 4.1 General
2 - 4.2 Peptone solution, 0.5%
2 - 4.3 Tryptone bile agar (TBA)
2 - 4.4 Minerals modified glutamate agar (MMGA)
2 - 4.5 Indole reagent (Vracko and Sherris)
2 - 5 REFERENCE CULTURES
2 - 5.1 Escherichia coli
2 - 5.2 Enterobacter aerogenes
3 - 6 APPARATUS
3 - 6.1 Cellulose ester membranes
3 - 6.2 Ultraviolet lamp
3 - 7 PROCEDURE
3 - 7.1 General
3 - 7.2 Resuscitation
4 - 7.3 Isolation
4 - 8 CALCULATION
4 - 9 REPORT
4 - 10 BIBLIOGRAPHY

This Standard sets out a rapid method for enumerating Escherichia coli in bivalve molluscsusing a membrane plate technique. This method will detect both typical E. coli and lactosenon-fermenting or anaerogenic variants.

This Standard sets out a rapid method for enumerating Escherichia coli in bivalve molluscs using a membrane plate technique. This method will detect both typical E. coli and lactose non-fermenting or anaerogenic variants.NOTES: 1 Other methods for examination of foods, including molluscs, for coliforms and E.coli are given in AS 1766.2.3.2 A flow diagram of the examination procedure is shown in Figure 1.

First published in part as AS 1766.2.12-1984.
AS 1095.3.10 first published 1984.
AS 1766.2.12-1984 and AS 1095.3.10-1984 revised, amalgamated and designated AS 1766.2.12-1994.
Jointly revised and designated AS/NZS 1766.2.12:2002.