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AS/NZS 1766.2.16.1:1998

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Food microbiology Examination for specific organisms - Food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes - Detection method

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Superseded date

30-01-2024

Language(s)

English

Published date

05-02-1998

€57.40
Excluding VAT

1 - AS/NZS 1766.2.16.1:1998 FOOD MICROBIOLOGY - EXAMINATION FOR SPECIFIC ORGANISMS-FOOD AND ANIMAL FEEDING STUFFS-HORIZONTAL ....
1 - PREFACE
2 - CONTENTS
3 - 1 Scope
3 - 2 Normative references
3 - 3 Definitions
3 - 3.1 Listeria monocytogenes:
3 - 3.2 detection of Listeria monocytogenes:
4 - 4 Principle
4 - 4.1 Primary enrichment in a selective liquid enrichment medium with reduced concentration of selective agents (half Fraser broth
4 - 4.2 Secondary enrichment with a selective liquid enrichment medium with full concentration of selective agents (Fraser broth)
4 - 4.3 Plating out and identification
4 - 4.4 Confirmation
4 - 5 Culture media and reagents
5 - 6 Apparatus and glassware
5 - 6.1 Apparatus for dry sterilization (oven) or wet sterilization (autoclave)
5 - 6.2 Drying cabinet or incubator,
5 - 6.3 Incubators,
5 - 6.4 Water bath,
5 - 6.5 Loops,
5 - 6.6 pH-meter,
5 - 6.7 Test tubes or flasks,
5 - 6.8 Measuring cylinders,
5 - 6.9 Total-delivery graduated pipettes,
5 - 6.10 Petri dishes,
5 - 6.11 Jars,
5 - 6.12 Gas mixture
5 - 6.13 Equipment for the Henry illumination test (optional).
5 - 6.14 Microscope,
5 - 7 Sampling
5 - 8 Preparation of test sample
5 - 9 Procedure
5 - 9.1 Test portion and initial suspension
6 - 9.2 Primary enrichment
6 - 9.3 Secondary enrichment
6 - 9.4 Plating out and identification
6 - 9.5 Confirmation of Listeria spp.
6 - 9.5.1 Selection of colonies for confirmation
7 - 9.5.2 Catalase reaction
7 - 9.5.3 Gram staining
7 - 9.5.4 Motility test (if necessary)
7 - 9.6 Confirmation of L. monocytogenes
7 - 9.6.1 Haemolysis test
7 - 9.6.2 Carbohydrate utilization
7 - 9.6.3 CAMP test
8 - 9.7 Interpretation of morphological and physiological properties and of the biochemical reactions
8 - 9.8 Definitive confirmation
9 - 9.9 Control cultures
9 - 10 Expression of results
9 - 11 Test report
10 - Annex A - Diagram of procedure
11 - Annex B - Composition and preparation of culture media and reagents
11 - B.1 Selective primary enrichment medium: Halt Fraser broth
12 - B.2 Selective secondary enrichment medium: Fraser broth
13 - B.3 First selective plating-out medium: Oxford agar
13 - B.4 Second selective plating-out medium: PALCAM agar
14 - B.5 Solid culture medium: Tryptone soya yeast extract agar (TSYEA)
15 - B.6 Liquid culture medium: Tryptone soya yeast extract broth (TSYEB)
15 - B.7 Sheep blood agar
15 - B.8 Carbohydrate utilization broth (rhamnose and xylose)
16 - B.9 Motility agar
16 - B.10 CAMP (Christie, Atkins, Munch-Petersen) medium and test strains
16 - B.11 Hydrogen peroxide solution
17 - B.12 Phosphate-buffered saline (PBS)
18 - Annex C - HENRY ILLUMINATION TEST
19 - ANNEX ZZ - ADDITIONAL REQUIREMENTS TO ISO 11290-1:1996 FOR AUSTRALIAN/NEW ZEALAND CONDITIONS
19 - ZZ1 SCOPE
19 - ZZ2 ADDITIONAL REQUIREMENTS

This part specifies a horizontal method for the detection of Listeria monocytogenes.

Committee
FT-024
DocumentType
Standard
ISBN
0 7337 1738 1
Pages
17
PublisherName
Standards Australia
Status
Superseded
SupersededBy
Supersedes
UnderRevision

This part of ISO 11290 specifies a horizontal method for the detection of Listeria monocytogenes.Subject to the limitations discussed in the introduction, this part of ISO 11290 is applicable to products intended for human consumption or animal feeding.

Standards Relationship
ISO 11290-1:1996 Identical

First published as AS/NZS 1766.2.16.1:1998.

AS 1766.3.1-1991 Food microbiology Examination of specific products - Meat and meat products other than poultry
AS 1766.1-1975 Methods for the microbiological examination of food General procedures and techniques

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