AS/NZS 4276.15:1999

1 - AS/NZS 4276.15:1999 WATER MICROBIOLOGY - EXAMINATION FOR VIBRIO CHOLERAE
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
2 - 2 PRINCIPLE
2 - 3 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
2 - 3.1 Culture media
2 - 3.2 Reagents
3 - 3.3 Reference cultures
3 - 4 SAMPLES
3 - 4.1 Sample volume
3 - 4.2 Concentration techniques
4 - 5 PROCEDURE
4 - 5.1 Enrichment
4 - 5.2 Selective agar cultures
4 - 5.3 Presumptive identification of Vibrio cholerae
5 - 5.4 Confirmation of Vibrio cholerae
6 - 6 TEST REPORT
7 - 7 ENUMERATION PROCEDURES
8 - 8 BIBLIOGRAPHY
9 - APPENDIX A - FLOW CHART FOR TESTING
10 - APPENDIX B - CULTURE MEDIA
10 - B1 SCOPE
10 - B2 0%, 3%, 8% NaCl TRYPTONE WATER
10 - B3 ALKALINE PEPTONE WATER
10 - B4 BUFFERED GLUCOSE BROTH
11 - B5 CARBOHYDRATE FERMENTATION BROTHS
11 - B5.1 Preparation of complete medium
11 - B5.2 Fermentation Broth base
11 - B5.3 Andrade's indicator
11 - B6 DECARBOXYLASE BROTH (Moeller's)
11 - B6.1 Preparation of complete medium
12 - B6.2 Decarboxylase broth base (Moeller's)
12 - B7 NITRATE REDUCTION BROTH
12 - B8 THIOSULFATE CITRATE BILE SALTS SUCROSE AGAR
13 - B9 TRYPTONE WATER
13 - B10 ONPG BROTH
13 - B10.1 Preparation of complete medium
13 - B10.2 Preparation of ONPG solution
14 - APPENDIX C - PERFORMANCE OF CONVENTIONAL TESTS REQUIRING ADDITION OF REAGENTS
14 - C1 INDOLE TEST
14 - C2 VOGES-PROSKAUER TEST (FOR THE PRODUCTION OF ACETOIN)
14 - C3 NITRATE REDUCTION TEST (Barritt)

This Standard sets out a method for detecting Vibrio cholerae in water using culture inenrichment broth and on selective agar media.

This Standard sets out a method for detecting Vibrio cholerae in water using culture in enrichment broth and on selective agar media.NOTES: 1 A flow diagram of the procedure is shown in Appendix A.2 This method is suitable for the isolation of other Vibrio species besides V. cholerae. However, the NaCl concentration in the enrichment broth should be increased to 1%.

First published as AS/NZS 4276.15:1999.