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AS/NZS 4276.17.1:2000

AS/NZS 4276.17.1:2000

AvailableSuperseded

AvailableSuperseded

An Available Superseded Standard is one, which has been made available for a period of time although it has been formally superseded by another Standard. Its availability is maintained where it is referenced in legislation/regulations or in other Standards and its use should be restricted to where so referenced. No responsibility is taken by SAI Global as to the ongoing technical validity of such a Standard, the responsibility resting with the referencing authority.

Water microbiology Spores of sulfite-reducing anaerobes (clostridia) including filtration method

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English

Published date

20-11-2000

Publisher

Standards Australia

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Hardcopy - English
PDF 1 User - English
PDF 3 Users - English
PDF 5 Users - English
PDF 9 Users - English
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1 - AS/NZS 4276.17.1:2000 WATER MICROBIOLOGY - SPORES OF SULFITE- REDUCING ANAEROBES (CLOSTRIDIA) INCLUDING CLOSTRIDIUM PERFRINGE
1 - PREFACE
2 - METHOD
2 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 PRINCIPLE
2 - 4 MEDIA, REAGENTS, AND REFERENCE CULTURES
2 - 4.1 Culture media (see AS 4276.2 and Appendix B).
2 - 4.2 Nitrate detection reagents (see Appendix B)
3 - 4.3 Reference culture
3 - 5 APPARATUS
3 - 5.1 Filtration equipment and 0.45 micrometre membranes
3 - 5.2 Anaerobic incubator/ jar
3 - 5.3 Incubator or water bath
3 - 6 TEST PROCEDURES
3 - 6.1 Membrane filtration method
4 - 7 CONFIRMATION OF C.perfringens
5 - 8 REPORT
5 - 9 BIBLIOGRAPHY
6 - APPENDIX A - FLOW CHART OF MEMBRANE FILTRATION PROCEDURES FOR SPORES OF SULFITE- REDUCING CLOSTRIDIA AND CLOSTRIDIUM PERFRING
7 - APPENDIX B - CULTURE MEDIA
7 - B1 SCOPE
7 - B2 TRYPTOSE SULFITE CYCLOSERINE AGAR
7 - B3 LACTOSE GELATIN MEDIUM
8 - B4 NITRATE MOTILITY MEDIUM
8 - B5 NITRITE DETECTION REAGENTS
8 - B5.1 Solution A, Sulfanilic acid reagent
8 - B5.2 Solution B, N-( 1- naphthyl) ethylenediamine reagent

This Standard sets out a method for enumerating spores of sulfite-reducing clostridia and C.perfringens in water, using membrane filtration, with a confirmation procedure for identifying C.perfringen.

Committee
FT-020
DocumentType
Standard
ISBN
0 7337 3528 2
Pages
7
PublisherName
Standards Australia
Status
AvailableSuperseded
SupersededBy
Supersedes
UnderRevision

This Standard sets out a method for enumerating spores of sulfite-reducing clostridia andC.perfringens in water, using membrane filtration, with a confirmation procedure foridentifying C.perfringens.Refer to AS 4276.1 for a description of the general procedure for determining counts ofmicroorganisms by membrane filtration techniques.NOTES:1 Membrane filtration is suitable for enumerating microorganisms only when the turbidity ofthe water is low.2 The membrane filtration method is not suitable for waters containing only small numbers ofC.perfringens in the presence of many other species that are capable of growth on the mediumused.3 Bacterial counts derived from membranes with fewer than 20 or more than 80 colonies areapproximate.4 A flow diagram of the procedure is shown in Appendix A.

First published as AS/NZS 4276.17.1:2000.

AS 4276.2-1995 Water microbiology Culture media, diluents and reagents (Reconfirmed 2013)
AS 4276.1-1995 Water microbiology General information and procedures

AS 4276.14:2014 Water microbiology Detection of Salmonella spp. (ISO 19250:2010, MOD)

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