AS/NZS 4276.17.1:2000

1 - AS/NZS 4276.17.1:2000 WATER MICROBIOLOGY - SPORES OF SULFITE- REDUCING ANAEROBES (CLOSTRIDIA) INCLUDING CLOSTRIDIUM PERFRINGE
1 - PREFACE
2 - METHOD
2 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 PRINCIPLE
2 - 4 MEDIA, REAGENTS, AND REFERENCE CULTURES
2 - 4.1 Culture media (see AS 4276.2 and Appendix B).
2 - 4.2 Nitrate detection reagents (see Appendix B)
3 - 4.3 Reference culture
3 - 5 APPARATUS
3 - 5.1 Filtration equipment and 0.45 micrometre membranes
3 - 5.2 Anaerobic incubator/ jar
3 - 5.3 Incubator or water bath
3 - 6 TEST PROCEDURES
3 - 6.1 Membrane filtration method
4 - 7 CONFIRMATION OF C.perfringens
5 - 8 REPORT
5 - 9 BIBLIOGRAPHY
6 - APPENDIX A - FLOW CHART OF MEMBRANE FILTRATION PROCEDURES FOR SPORES OF SULFITE- REDUCING CLOSTRIDIA AND CLOSTRIDIUM PERFRING
7 - APPENDIX B - CULTURE MEDIA
7 - B1 SCOPE
7 - B2 TRYPTOSE SULFITE CYCLOSERINE AGAR
7 - B3 LACTOSE GELATIN MEDIUM
8 - B4 NITRATE MOTILITY MEDIUM
8 - B5 NITRITE DETECTION REAGENTS
8 - B5.1 Solution A, Sulfanilic acid reagent
8 - B5.2 Solution B, N-( 1- naphthyl) ethylenediamine reagent

This Standard sets out a method for enumerating spores of sulfite-reducing clostridia and C.perfringens in water, using membrane filtration, with a confirmation procedure for identifying C.perfringen.

This Standard sets out a method for enumerating spores of sulfite-reducing clostridia andC.perfringens in water, using membrane filtration, with a confirmation procedure foridentifying C.perfringens.Refer to AS 4276.1 for a description of the general procedure for determining counts ofmicroorganisms by membrane filtration techniques.NOTES:1 Membrane filtration is suitable for enumerating microorganisms only when the turbidity ofthe water is low.2 The membrane filtration method is not suitable for waters containing only small numbers ofC.perfringens in the presence of many other species that are capable of growth on the mediumused.3 Bacterial counts derived from membranes with fewer than 20 or more than 80 colonies areapproximate.4 A flow diagram of the procedure is shown in Appendix A.

First published as AS/NZS 4276.17.1:2000.