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AS/NZS 4276.17.2:2000

AS/NZS 4276.17.2:2000

AvailableSuperseded

AvailableSuperseded

An Available Superseded Standard is one, which has been made available for a period of time although it has been formally superseded by another Standard. Its availability is maintained where it is referenced in legislation/regulations or in other Standards and its use should be restricted to where so referenced. No responsibility is taken by SAI Global as to the ongoing technical validity of such a Standard, the responsibility resting with the referencing authority.

Water microbiology Spores of sulfite-reducing anaerobes (clostridia) including - Estimation of most probable number (MPN) using the multiple tube dilution technique

Available format(s)

Hardcopy , PDF 1 User , PDF 3 Users , PDF 5 Users , PDF 9 Users

Language(s)

English

Published date

20-11-2000

Publisher

Standards Australia

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Hardcopy - English
PDF 1 User - English
PDF 3 Users - English
PDF 5 Users - English
PDF 9 Users - English
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1 - AS/NZS 4276.17.2:2000 WATER MICROBIOLOGY - SPORES OF SULFITE-REDUCING ANAEROBES (CLOSTRIDIA) INCLUDING CLOSTRIDIUM PERFRINGEN
1 - PREFACE
2 - METHOD
2 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 PRINCIPLE
2 - 4 MEDIA, REAGENTS, AND REFERENCE CULTURES
2 - 4.1 Culture media (see AS 4276.2 and Appendix B)
2 - 4.2 Nitrite detection reagents (see Appendix B)
2 - 4.3 Reference culture
3 - 5 APPARATUS
3 - 5.1 Suitable screw cap containers for the volume of water to be tested
3 - 5.2 Pipettes or equivalent measuring devices
3 - 5.3 Incubators and water baths
3 - 6 TEST PROCEDURES
3 - 6.1 MPN by multiple tube dilution technique
4 - 7 CONFIRMATION OF C.perfringens
4 - 8 REPORT
5 - 9 BIBLIOGRAPHY
6 - APPENDIX A - FLOW CHART OF MPN EXAMINATION PROCEDURES FOR SPORES OF SULFITE- REDUCING CLOSTRIDIA AND CLOSTRIDIUM PERFRINGENS
7 - APPENDIX B - CULTURE MEDIA
7 - B1 SCOPE
7 - B2 DIFFERENTIAL REINFORCED CLOSTRIDIAL MEDIUM (single strength)
7 - B3 TRYPTOSE SULFITE CYCLOSERINE AGAR
8 - B4 LACTOSE GELATIN MEDIUM
8 - B5 NITRATE MOTILITY MEDIUM
8 - B6 NITRITE DETECTION REAGENTS
8 - B6.1 Solution A, Sulfanilic acid reagent
9 - B6.2 Solution B, N-( 1- naphthyl) ethylenediamine reagent

This Standard sets out a method for determining the most probable number (MPN) of spores of sulfite-reducing anaerobes (clostridia) including C.perfringens in water, using a multiple tube dilution technique, with a confirmation procedure for identifying C.perfringens.

Committee
FT-020
DocumentType
Standard
ISBN
0 7337 3529 0
Pages
8
PublisherName
Standards Australia
Status
AvailableSuperseded
SupersededBy
Supersedes

This Standard sets out a method for determining the most probable number (MPN) of sporesof sulfite-reducing anaerobes (clostridia) including C.perfringens in water, using a multipletube dilution technique, with a confirmation procedure for identifying C.perfringens.Refer to AS 4276.1 for a description of the general procedure for estimating the MPN ofmicroorganisms.MPN procedures may be used for the examination of all water samples and are particularlyapplicable for samples containing sufficient suspended material to make filtrationprocedures impractical.NOTE: A flow diagram of the procedure is shown in Appendix A.

First published as AS/NZS 4276.17.2:2000.

AS 4276.2-1995 Water microbiology Culture media, diluents and reagents (Reconfirmed 2013)
AS 4276.1-1995 Water microbiology General information and procedures

AS 4276.14:2014 Water microbiology Detection of Salmonella spp. (ISO 19250:2010, MOD)

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