AS/NZS 4276.19:2001

1 - AS/NZS 4276.19:2001 WATER MICROBIOLOGY - EXAMINATION FOR THERMOPHILIC CAMPYLOBACTER SPP.-MEMBRANE FILTRATION
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
1 - 2 REFERENCED DOCUMENT
2 - 3 PRINCIPLE
2 - 4 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
2 - 4.1 Culture media
2 - 4.1.1 Preston enrichment broth
2 - 4.1.2 Prestons selective agar or a blood free modification of this medium
2 - 4.1.3 Blood agar
2 - 4.2 Reagent
2 - 4.3 Reference cultures
2 - 4.3.1 Campylobacter jejuni
2 - 4.3.2 Escherichia coli
2 - 4.3.3 Use of reference cultures
2 - 5 APPARATUS
2 - 5.1 Gas cylinders or kits
3 - 5.2 Filtration equipment and 0.2 µm or 0.45 µm membranes
3 - 5.3 Incubators
3 - 6 PROCEDURES
3 - 6.1 Micro-aerobic incubation conditions
3 - 6.2 Sample volume
3 - 6.3 Membrane filtration
3 - 6.4 Enrichment
3 - 6.5 Controls for broth culture
3 - 6.6 Selective enrichment
3 - 6.7 Plating out on selective agar media
3 - 6.8 Examination of selective agar plates
4 - 7 CONFIRMATION TESTS
4 - 7.1 Culture of selected colonies
4 - 7.2 Oxidase test and Gram stain
4 - 7.3 Further confirmation tests
4 - 8 TEST REPORT
5 - 9 BIBLIOGRAPHY
6 - APPENDIX A - FLOW CHART FOR EXAMINATION FOR THERMOPHILIC CAMPYLOBACTER SPP.-MEMBRANE FILTRATION
7 - APPENDIX B - CULTURE MEDIA
7 - B1 SCOPE
7 - B2 BLOOD AGAR
7 - B2.1 General
7 - B2.2 Nutrient agar
7 - B3 PRESTON ENRICHMENT BROTH
7 - B3.1 General
8 - B3.2 Nutrient broth
8 - B3.3 FBP Supplement
8 - B3.4 Antibiotic supplement
8 - B4 PRESTON SELECTIVE AGAR
9 - B5 CAMPYLOBACTER BLOOD- FREE SELECTIVE MEDIUM (MODIFIED CCDA- PRESTONS)
9 - B6 CARBOL FUCHSIN
9 - B6.1 Strong
9 - B6.2 Weak

This Standard sets out a method for detecting thermophilic Campylobacters in water using culture in enrichment broth and on selective agar media.

This Standard sets out a method for detecting thermophilic Campylobacters in water using culture in enrichment broth and on selective agar media.NOTE: A flow diagram of the procedure is shown in Appendix A.

First published as AS/NZS 4276.19:2001.