AS/NZS 4276.20:2003

1 - AS/NZS 4276.20:2003 WATER MICROBIOLOGY - EXAMINATION FOR COAGULASE POSITIVE STAPHYLOCOCCI, INCLUDING STAPHYLOCOCCUS...
1 - PREFACE
1 - METHOD
1 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 PRINCIPLE
2 - 4 CULTURE MEDIA, REAGENTS AND REFERENCE CULTURES
2 - 4.1 Culture media
2 - 4.1.1 Baird-Parker agar (BP)
2 - 4.1.2 Brain heart infusion (BHI) broth
2 - 4.1.3 Nutrient agar
2 - 4.2 Reagents (see AS 4276.2)
2 - 4.2.1 Saline solution, 0.85%
2 - 4.2.2 Rabbit blood plasma-
2 - 4.3 Reference cultures
2 - 4.3.1 Positive culture
2 - 4.3.2 Negative culture
2 - 4.3.3 Use of reference cultures
3 - 5 SAMPLES
3 - 5.1 Laboratory samples
3 - 5.2 Dilutions
3 - 6 PROCEDURE
3 - 6.1 Membrane filtration
3 - 6.2 Presumptive identification of coagulase positive staphylococci
3 - 6.3 Confirmation of coagulase positive staphylococci
5 - 6.4 Confirmation of Staphylococcus aureus
5 - 7 CALCULATION OF RESULT
6 - 8 TEST REPORT
6 - 9 BIBLIOGRAPHY
7 - APPENDIX A - FLOW CHART FOR STAPHYLOCOCCI TESTING
8 - APPENDIX B - CULTURE MEDIA
8 - B1 SCOPE
8 - B2 BAIRD-PARKER AGAR
8 - B2.1 Preparation of complete medium
8 - B2.2 Basal medium
9 - B2.3 Concentrated egg yolk emulsion
9 - B2.4 Solutions
9 - B3 BRAIN HEART INFUSION BROTH
9 - B3.1 Formulation
9 - B3.2 Preparation
10 - B3.3 Preparation of calf brain infusion
10 - B3.4 Preparation of beef heart infusion

This Standard sets out a method for detecting coagulase positive staphylococci and Staphylococcus aureus (S.aureus) in water by the membrane filtration method.

This Standard sets out a method for detecting coagulase positive staphylococci and Staphylococcus aureus (S.aureus) in water by the membrane filtration method. NOTES: 1 Membrane filtration is suitable for enumerating microorganisms only when the turbidity of the water is low. 2 The optimum range for membrane filter counts is 20-80 colonies. If the confirmed total count from one or more membranes is less than 20, then the derived sample count, which is an estimate of the true count in the sample (population parameter), is regarded as having a low precision. At the other extreme, when membranes containing greater than 80 colonies are counted, the counting error tends to be large. Again, the derived counts are regarded as having low precision. Derived counts of low precision should be indicated on reports by flagging them as ‘estimated’ or ‘approximate’. 3 A flow diagram of the procedure is shown in Appendix A.

First published as AS/NZS 4276.20:2003.