AS/NZS 4276.6:2007

1 - AS/NZS 4276.6:2007 WATER MICROBIOLOGY - METHOD 6: COLIFORMS, ESCHERICHIA COLI AND THERMOTOLERANT COLIFORMS-DETERMINATION OF M
1 - PREFACE
2 - METHOD
2 - 1 SCOPE
2 - 2 REFERENCED DOCUMENTS
2 - 3 DEFINITIONS
2 - 3.1 Coliforms
2 - 3.2 Thermotolerant coliforms
2 - 3.3 Escherichia coli (E. coli)
2 - 4 PRINCIPLE
3 - 5 CULTURE MEDIA AND REAGENTS (See Appendix B)
3 - 6 APPARATUS
3 - 7 REFERENCE CULTURES
3 - 7.1 Escherichia coli, NCTC 9001 or ATCC 11775 or ACM 1803 or NZRM 3309 or a culture traceable to any of these strains
3 - 7.2 Enterobacter aerogenes, NCTC 10006 or ATCC 13048 or ACM 4982 or NZRM 798 or a culture traceable to any of these strains
3 - 7.3 Pseudomonas aeruginosa, NCTC 10332 or ATCC 10145 or ACM 495 or NZRM 3007 or a culture traceable to any of these strains
3 - 7.4 Use of reference cultures
4 - 8 SAMPLES
4 - 8.1 Laboratory samples
4 - 8.2 Dilutions
4 - 9 PROCEDURE
4 - 9.1 Presumptive test
5 - 9.2 Confirmatory tests
5 - 9.2.1 Coliforms
5 - 9.2.2 E. coli
6 - 9.2.3 Thermotolerant coliforms
6 - 10 DETERMINATION OF THE MOST PROBABLE NUMBER (MPN) OF COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS
6 - 11 TEST REPORT
6 - 12 BIBLIOGRAPHY
7 - APPENDIX A - FLOW DIAGRAM FOR COLIFORMS, E. COLI AND THERMOTOLERANT COLIFORMS-MOST PROBABLE NUMBER
8 - APPENDIX B - CULTURE MEDIA AND REAGENTS
8 - B1 SCOPE
8 - B2 IMPROVED FORMATE LACTOSE GLUTAMATE MEDIUM
10 - B3 ORTHO-NITROPHENYL-β D-GALACTOPYRANOSIDE (ONPG) BROTH
10 - B4 NUTRIENT AGAR
11 - B5 MACCONKEY AGAR
11 - B6 OXIDASE REAGENT (KOVACS’)
11 - B7 EC-MUG BROTH
12 - APPENDIX C - PERFORMANCE OF KOVACS’ OXIDASE TEST

This Standard sets out a method, using a multiple tube dilution technique, for determining the most probable number (MPN) of coliforms, Escherichia coli (E. coli) and thermotolerant coliforms in water. It is suitable for waters with high background flora and/or turbidity.

This Standard sets out a method, using a multiple tube dilution technique, for determining the most probable number (MPN) of coliforms, Escherichia coli (E. coli) and thermotolerant coliforms in water. It is suitable for waters with high background flora and/or turbidity.The method is suitable for non-food solid matrices. The preparation of such matrices are beyond the scope of this method (see Clause 12, Bibliography for further guidance).NOTES:1 Thermotolerant coliforms are also referred to as faecal coliforms although the organisms may not be of faecal origin. 2 A flow diagram of the procedure is shown in Appendix A.

Originated as AS 1095.4.1.3-1981 and AS 1095.4.1.4-1981.
Previous editions AS 4276.4-1995 and AS 4276.6-1995.
AS 4276.4-1995 and AS 4276.6-1995 jointly revised, amalgamated and designated as AS/NZS 4276.6:2007.