• ASTM D 5712 : 2010

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

    Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method

    Available format(s):  Hardcopy, PDF

    Superseded date:  11-11-2014

    Language(s):  English

    Published date:  01-06-2010

    Publisher:  American Society for Testing and Materials

    Add To Cart

    Scope - (Show below) - (Hide below)

    1.1 This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with natural rubber (NR) and its products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

    1.2 For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm2 NR specimen.

    1.3 The test method is designed to be accurate and compatible with the industrial environment.

    1.4 Steps are included in this test method to minimize the effects of interfering substances.

    1.5 It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this test method.

    1.6 This test method has not been validated for use with lubricated products such as condoms. Condoms with different lubricants as typically marketed, have not been tested in an ASTM ILS to determine if, and if so to what degree, the lubricant interferes with the assay.

    1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    General Product Information - (Show below) - (Hide below)

    Committee D 11
    Document Type Test Method
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM D 3578 : 2005 : R2015 Standard Specification for Rubber Examination Gloves
    ASTM D 7329 : 2007 : R2018 Standard Specification for Food Preparation and Food Handling (Food Service) Gloves
    ASTM D 7198 : 2005 : R2018 Standard Specification for Disposable Embalming Gloves for Single-Use Applications
    ASTM D 3577 : 2009 : R2015 Standard Specification for Rubber Surgical Gloves
    ASTM D 6499 : 2018 Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products
    ASTM D 7427 : 2016 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex
    ASTM D 7103 : 2018 Standard Guide for Assessment of Medical Gloves
    ASTM D 1076 : 2015 Standard Specification for Rubber—Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex
    ASTM D 7850 : 2018 Standard Specification for Biological Properties of Industry Reference Materials (IRM)
    • Access your standards online with a subscription

      Features

      • Simple online access to standards, technical information and regulations
      • Critical updates of standards and customisable alerts and notifications
      • Multi - user online standards collection: secure, flexibile and cost effective