• ASTM D 6499 : 2000

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

    Standard Test Method for the Immunological Measurement of Antigenic Protein in Natural Rubber and its Products

    Available format(s):  Hardcopy, PDF

    Superseded date:  11-11-2014

    Language(s):  English

    Published date:  10-01-2000

    Publisher:  American Society for Testing and Materials

    Add To Cart

    Scope - (Show below) - (Hide below)

    1.1 This test method covers an immunological method to determine the amount of antigenic protein in natural rubber and its products using rabbit antisera specific for natural rubber latex (NRL) proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other rubber product extracts which have been collected in order to measure the latex protein levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

    1.2 For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

    This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    General Product Information - (Show below) - (Hide below)

    Committee D 11
    Document Type Test Method
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM D 3578 : 2005 : R2015 Standard Specification for Rubber Examination Gloves
    ASTM D 8243 : 2019 Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method
    ASTM D 7198 : 2005 : R2018 Standard Specification for Disposable Embalming Gloves for Single-Use Applications
    ASTM D 7329 : 2007 : R2018 Standard Specification for Food Preparation and Food Handling (Food Service) Gloves
    ASTM D 3577 : 2009 : R2015 Standard Specification for Rubber Surgical Gloves
    ASTM D 7427 : 2016 Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex
    ASTM D 7103 : 2018 Standard Guide for Assessment of Medical Gloves
    ASTM D 1076 : 2015 Standard Specification for Rubber—Concentrated, Ammonia Preserved, Creamed, and Centrifuged Natural Latex
    ASTM D 7850 : 2018 Standard Specification for Biological Properties of Industry Reference Materials (IRM)
    • Access your standards online with a subscription

      Features

      • Simple online access to standards, technical information and regulations
      • Critical updates of standards and customisable alerts and notifications
      • Multi - user online standards collection: secure, flexibile and cost effective