ASTM D 7463 :2014

1.1This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with:

1.2The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens² and a dedicated luminometer² and reported in (instrument specific) Relative Light Units.

1.3This test method is equally suitable for use in the laboratory or field.

1.4Although bioluminescence is a reliable and proven method for qualifying and quantifying ATP, this method does not differentiate between ATP from different sources, for example, from different types of microorganism such as bacteria or fungi.

1.5For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 × 10^–11 M to 3 × 10^–8 M which is equivalent to 1 × 10^–14 moles/mL to 3 × 10^–11 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 × 10^–11 M to 2 × 10^–14 M.

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.