ASTM D 7463 : 2016 : EDT 1
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Fuel, Fuel/Water Mixtures, and Fuel Associated Water
Hardcopy , PDF
18-12-2018
English
01-06-2016
Important note : Users cannot be edited or removed once added to your Multi user PDF.| Committee |
D 02
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| DocumentType |
Test Method
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| Pages |
8
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| PublisherName |
American Society for Testing and Materials
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| Status |
Superseded
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| SupersededBy | |
| Supersedes |
1.1This test method provides a protocol for capturing, concentrating, and testing the adenosine triphosphate (ATP) present in a fuel system sub-sample (that is, test specimen) associated with:
1.1.1Microorganisms and hydrophilic particles found in liquid fuels as described in Table X6.1, or
1.1.2Microorganisms and hydrophilic particles found in mixture of fuel and associated bottom water or just associated bottom water.
1.1.3ATP detected by this bioluminescence test can be derived from cellular ATP, extra-cellular ATP, or some combination of both.
1.1.4Cellular and extra-cellular ATP utilized to perform ATP bioluminescence are captured and concentrated from a fuel system sample into an aqueous test specimen (that is, sub-sample) for testing. For example, for a fuel system sample that does not contain any visible fuel associated bottom water, the aqueous test specimen is the capture solution itself described in 8.2.1.1. For fuel system samples that are a mixture of fuel and associated bottom water (that is, free water), the test specimen is an aliquant of the capture solution and associated bottom water.
1.2The ATP is measured using a patented bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the sample. The light is produced and measured quantitatively using dedicated ATP test pens2 and a dedicated luminometer2 and reported in (instrument specific) Relative Light Units.
1.3This test method is equally suitable for use in the laboratory or field.
1.4Although bioluminescence is a reliable and proven technology, this method does not differentiate ATP from bacteria or fungi.
1.5For water or capture solution samples, the concentration range of ATP detectable by this test method is 1 × 10–11 M to 3 × 10–8 M which is equivalent to 1 × 10–14 moles/mL to 3 × 10–11 moles/mL for water samples or capture solution. Assuming testing on fuel phase is performed on a 500 mL volume of fuel the equivalent concentrations is fuel would be: 6 × 10–11 M to 2 × 10–14 M.
1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.6.1There is one exception—Relative Light Unit (RLU) as defined in 3.1.19.
1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
| ASTM E 1259 : 2018 | Standard Practice for Evaluation of Antimicrobials in Liquid Fuels Boiling Below 390 °C |
| ASTM D 7687 : 2017 | Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration |
| ASTM D 7847 : 2017 | Standard Guide for Interlaboratory Studies for Microbiological Test Methods |
| ASTM D 6469 : 2017 | Standard Guide for Microbial Contamination in Fuels and Fuel Systems |
| ASTM D 6974 : 2016 | Standard Practice for Enumeration of Viable Bacteria and Fungi in Liquid Fuels—Filtration and Culture Procedures |
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