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Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks (Withdrawn 2022)
:
Available format(s): Hardcopy, PDF
Superseded date: 28-06-2022
Language(s): English
Published date: 21-03-2013
Publisher: American Society for Testing and Materials
CONTAINED IN VOL. 11.06, 2013 Defines procedures for obtaining laboratory data concerning bioconcentration of a test material added to dilution water-but not to food-by freshwater and saltwater fishes and saltwater bivalve mollusks using the flow-through technique.
1.1This guide describes procedures for obtaining laboratory data concerning bioconcentration of a test material added to dilution water—but not to food—by freshwater and saltwater fishes and saltwater bivalve mollusks using the flow-through technique. These procedures also should be useful for conducting bioconcentration tests with other aquatic species, although modifications might be necessary.
1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. The comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting bioconcentration tests.
1.3These procedures are applicable to all chemicals that can be measured accurately at the necessary concentrations in water and in appropriate tissues. Bioconcentration tests are usually conducted on individual chemicals but can be conducted on mixtures if appropriate measurements can be made. Some techniques described in this guide were developed for tests on non-ionizable organic chemicals (see 11.1.2.1) and might not apply to ionizable or inorganic chemicals.
1.4Results of bioconcentration tests should usually be reported in terms of apparent steady-state and projected steady-state bioconcentration factors (BCFs) and uptake and depuration rate constants. Results should be reported in terms of whole body for fishes and in terms of total soft tissue for bivalve mollusks. For fishes and scallops consumed by humans, some results should also be reported in terms of the edible portion, especially if ingestion of the test material by humans is a major concern. For tests on organic and organometallic chemicals, the percent lipids of the tissue should be reported.
1.5This guide is arranged as follows:
Section
Referenced Documents
2
Terminology
3
Summary of Guide
4
Significance and Use
5
Safety Precautions
7
Apparatus
6
Facilities
6.1
Construction Materials
6.2
Metering System
6.3
Test Chambers
6.4
Cleaning
6.4.4
Acceptability
6.5
Dilution Water
8
Requirements
8.1
Source
8.2
Treatment
8.3
Characterization
8.4
Test Material
9
General
9.1
Radiolabeled Material
9.2
Stock Solution
9.3
Test Concentration(s)
9.4
Test Organisms
10
Species
10.1
Size
10.2
10.3
Care and Handling
10.4
Feeding
10.5
Disease Treatment
10.6
Holding
10.7
Acclimation
10.8
Quality
10.9
Procedure
11
Experimental Design
11.1
Dissolved Oxygen
11.2
Temperature
11.3
Loading
11.4
Beginning the Test
11.5
Care of Organisms
11.6
11.7
11.8
Biological Data
11.9
Measurements on Test Solutions
11.10
Analytical Methodology
12
Acceptability of Test
13
Calculation of Results
14
Documentation
15
Keywords
16
1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.
1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.
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