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ASTM E 1192 : 1997 : R2014
Superseded
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Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
Hardcopy , PDF
16-03-2023
English
04-12-2014
CONTAINED IN VOL. 11.06, 2014 Defines procedures for obtaining laboratory data concerning the adverse effects of an aqueous effluent on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians, usually during 2 to 4-day exposures, depending on the species, using the static, renewal, and flow-through techniques.
Committee |
E 50
|
DocumentType |
Guide
|
Pages |
14
|
ProductNote |
Reconfirmed 2014
|
PublisherName |
American Society for Testing and Materials
|
Status |
Superseded
|
SupersededBy | |
Supersedes |
1.1This guide covers procedures for obtaining laboratory data concerning the adverse effects of an aqueous effluent on certain species of freshwater and saltwater fishes, macroinvertebrates, and amphibians, usually during 2 to 4-day exposures, depending on the species, using the static, renewal, and flow-through techniques. These procedures will probably be useful for conducting acute toxicity tests on aqueous effluents with many other aquatic species, although modifications might be necessary.
1.2Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute toxicity tests on aqueous effluents.
1.3This guide is based in large part on Guide E729. The major differences between the two guides are (1) the maximum test concentration is 100 % effluent or ambient sample, (2) testing is not chemical specific, and (3) the holding time of effluent and ambient samples is often considerably less than that for chemicals and other test materials. Because the sample is often a complex mixture of chemicals, analytical tests cannot generally be used to confirm exposure concentrations.
1.4Selection of the technique to be used in a specific situation will depend upon the needs of the investigator and upon available resources. Static tests provide the most easily obtained measure of acute toxicity, but should not last longer than 48 h. Renewal and flow-through tests may last longer than 48 h because the pH and concentrations of dissolved oxygen and effluent are maintained at desired levels and degradation and metabolic products are removed. Static tests might not be applicable to effluents that have a high oxygen demand, or contain materials that (1) are highly volatile, (2) are rapidly biologically or chemically transformed in aqueous solutions, or (3) are removed from test solutions in substantial quantities by the test chambers or organisms during the test. Flow-through tests are generally preferable to renewal tests, although in some situations a renewal test might be more cost-effective than a flow-through test.
1.5In the development of these procedures, an attempt was made to balance scientific and practical considerations and to ensure that the results will be sufficiently accurate and precise for the applications for which they are commonly used. A major consideration was that the common uses of the results of acute tests on effluents do not require or justify stricter requirements than those set forth in this guide. Although the tests may be improved by using more organisms, longer acclimation times, and so forth, the requirements presented in this guide should usually be sufficient.
1.6Results of acute toxicity tests should usually be reported in terms of a median lethal concentration (LC50) or median effective concentration (EC50). In some situations, it might be necessary only to determine whether a specific concentration is acutely toxic to the test species or whether the LC50 or EC50 is above or below a specific concentration.
1.7This guide is arranged as follows:
Section | |
Referenced Documents | 2 |
Terminology | 3 |
Summary of Guide | 4 |
Significance and Use | 5 |
Hazards | 7 |
Apparatus | 6 |
Facilities | 6.1 |
Special Requirements | 6.2 |
Construction Materials | 6.3 |
Metering System | 6.4 |
Test Chambers | 6.5 |
Cleaning | 6.6 |
Acceptability | 6.7 |
Dilution Water | 8 |
Requirements | 8.1 |
Source | 8.2 |
Treatment | 8.3 |
Characterization | 8.4 |
Effluent | 9 |
Sampling Point | 9.1 |
Collection | 9.2 |
Preservation | 9.3 |
Treatment | 9.4 |
Test Concentration(s) | 9.5 |
Test Organisms | 10 |
Species | 10.1 |
Age | 10.2 |
Source | 10.3 |
Care and Handling | 10.4 |
Feeding | 10.5 |
Disease Treatment | 10.6 |
Holding | 10.7 |
Acclimation | 10.8 |
Quality | 10.9 |
Procedure | 11 |
Experimental Design | 11.1 |
Dissolved Oxygen | 11.2 |
Temperature | 11.3 |
Loading | 11.4 |
Beginning the Test | 11.5 |
Feeding | 11.6 |
Duration of Test | 11.7 |
Biological Data | 11.8 |
Other Measurements | 11.9 |
Analytical Methodology | 12 |
Acceptability of Test | 13 |
Calculation or Results | 14 |
Report | 15 |
1.8This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 7.
ASTM E 729 : 2023 : EDT 1 | Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians |
ASTM E 1191 : 2003 : REV A : R2023 : EDT 1 | Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids |
ASTM E 1562 : 2022 | Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids |
ASTM E 1604 : 2020 | Standard Guide for Behavioral Testing in Aquatic Toxicology |
ASTM E 2591 : 2022 | Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians |
ASTM E 1241 : 2022 | Standard Guide for<brk type="line"/> Conducting Early Life-Stage Toxicity Tests with Fishes |
ASTM E 1022 : 2022 | Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks |
ASTM E 1218 : 2021 | Standard Guide for Conducting Static Toxicity Tests with Microalgae |
ASTM E 1563 : 2021 : REV A | Standard Guide for Conducting Short-Term Chronic Toxicity Tests with Echinoid Embryos |
ASTM E 1295 : 2022 | Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <emph type="ital">Ceriodaphnia dubia</emph> |
ASTM E 1611 : 2021 | Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids |
ASTM E 1415 : 2022 | Standard Guide for Conducting Static Toxicity Tests With <emph type="ital">Lemna gibba</emph> G3 |
ASTM E 1193 : 2020 | Standard Guide for Conducting <emph type="ital">Daphnia magna</emph> Life-Cycle Toxicity Tests |
ASTM E 1711 : 2020 | Standard Guide for Measurement of Behavior During Fish Toxicity Tests |
ASTM E 1295 : 2001 : R2013 | Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with <emph type="ital">Ceriodaphnia dubia</emph><emph type="bold"> |
ASTM E 1850 : 2004 : R2019 | Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests |
ASTM E 1611 : 2000 : R2013 | Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids |
ASTM E 1463 : 1992 : R2012 | Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States (Withdrawn 2021) |
ASTM E 1849 : 1996 : R2013 | Standard Guide for Fish and Wildlife Incident Monitoring and Reporting (Withdrawn 2022) |
ASTM E 1768 : 1995 : R2013 | Standard Guide for Ventilatory Behavioral Toxicology Testing of Freshwater Fish (Withdrawn 2022) |
ASTM E 1366 : 2011 : R2016 | Standard Practice for Standardized Aquatic Microcosms: Fresh Water |
ASTM E 1415 : 1991 : R2012 | Standard Guide for Conducting Static Toxicity Tests With <i>Lemna gibba</i> G3 (Withdrawn 2021) |
ASTM E 1241 : 2005 : R2013 | Standard Guide for<brk type="line"/> Conducting Early Life-Stage Toxicity Tests with Fishes (Withdrawn 2022) |
ASTM E 1498 : 1992 : R2012 | Standard Guide for Conducting Sexual Reproduction Tests with Seaweeds (Withdrawn 2021) |
ASTM E 2122 : 2002 : R2013 | Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves (Withdrawn 2022) |
ASTM E 1439 : 2012 : R2019 | Standard Guide for Conducting the Frog Embryo Teratogenesis Assay-Xenopus (FETAX) |
ASTM E 1193 : 1997 : R2012 | <i xmlns:a="http://library.astm.org" xmlns="">Daphnia magna</i> |
ASTM E 1191 : 2003 : REV A : R2014 | Standard Guide for Conducting Life-Cycle Toxicity Tests with Saltwater Mysids |
ASTM E 1563 : 1998 : R2012 | Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos |
ASTM E 1218 : 2004 : R2012 | Standard Guide for Conducting Static Toxicity Tests with Microalgae (Withdrawn 2021) |
ASTM E 2317 : 2004 : R2012 | Standard Guide for Conducting Renewal Microplate-Based Life-Cycle Toxicity Tests with a Marine Meiobenthic Copepod (Withdrawn 2021) |
ASTM E 724 : 1998 : R2012 | Standard Guide for<brk type="line"/> Conducting Static Acute Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs |
ASTM E 729 : 1996 : R2014 | Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians |
ASTM E 1841 : 2004 : R2012 | Standard Guide for Conducting Renewal Phytotoxicity Tests With Freshwater Emergent Macrophytes (Withdrawn 2021) |
ASTM E 1440 : 1991 : R2012 | Standard Guide for Acute Toxicity Test with the Rotifer Brachionus (Withdrawn 2021) |
ASTM E 1604 : 1994 : R2007 | Standard Guide for Behavioral Testing in Aquatic Toxicology |
ASTM E 1604 : 2012 | Standard Guide for Behavioral Testing in Aquatic Toxicology |
ASTM E 1604 : 2012 : REDLINE | Standard Guide for Behavioral Testing in Aquatic Toxicology |
IEEE/ASTM SI_10-2010 | American National Standard for Metric Practice |
ASTM E 724 : 2021 | Standard Guide for<brk type="line"/> Conducting Static Short-Term Chronic Toxicity Tests Starting with Embryos of Four Species of Saltwater Bivalve Molluscs |
ASTM E 1604 : 1994 : R2002 | Standard Guide for Behavioral Testing in Aquatic Toxicology |
ASTM E 1203 : 1998 : R2004 | Standard Practice for Using Brine Shrimp Nauplii as Food for Test Animals in Aquatic Toxicology |
ASTM E 943 : 2008 : R2014 | Standard Terminology Relating to Biological Effects and Environmental Fate (Withdrawn 2023) |
ASTM E 1203 : 1998 : R2012 | Standard Practice for<brk type="line"/> Using Brine Shrimp Nauplii as Food for Test Animals in Aquatic Toxicology (Withdrawn 2013) |
ASTM E 729 : 1996 | Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians |
ASTM E 1604 : 1994 | Standard Guide for Behavioral Testing in Aquatic Toxicology |
ASTM E 1203 : 1998 | Standard Practice for Using Brine Shrimp Nauplii as Food for Test Animals in Aquatic Toxicology |
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