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ASTM E 1611 : 2000 : R2013

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by

Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids

Available format(s)

Hardcopy , PDF

Superseded date

28-01-2022

Superseded by

ASTM E 1611 : 2021

Language(s)

English

Published date

25-03-2013

€96.91
Excluding VAT

CONTAINED IN VOL. 11.06, 2013 Defines procedures for obtaining laboratory data concerning the adverse effects of potentially contaminated sediment, or of a test material added experimentally to contaminated or uncontaminated sediment, on marine or estuarine infaunal polychaetes during 10-day or 20 to 28-day exposures.

Committee
E 50
DocumentType
Guide
Pages
26
ProductNote
Reconfirmed 2013
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

1.1This guide covers procedures for obtaining laboratory data concerning the adverse effects of potentially contaminated sediment, or of a test material added experimentally to contaminated or uncontaminated sediment, on marine or estuarine infaunal polychaetes during 10-day or 20 to 28-day exposures. These procedures are useful for testing the effects of various geochemical characteristics of sediments on marine and estuarine polychaetes and could be used to assess sediment toxicity to other infaunal taxa, although modifications of the procedures appropriate to the test species might be necessary. Procedures for the 10-day static test are described for Neanthes arenaceodentata and Neanthes virens and for the 20 to 28-day static-renewal sediment toxicity for N. arenaceodentata.

1.2Modifications of these procedures might be appropriate for other sediment toxicity test procedures, such as flow-through or partial life-cycle tests. The methods outlined in this guide should also be useful for conducting sediment toxicity tests with other aquatic taxa, although modifications might be necessary. Other test organisms might include other species of polychaetes, crustaceans, and bivalves.

1.3Other modifications of these procedures might be appropriate for special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sediment tests with infaunal organisms.

1.4These procedures are applicable to sediments contaminated with most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. These procedures can be used with appropriate modifications to conduct sediment toxicity tests on factors such as temperature, salinity, dissolved oxygen (DO), and natural sediment characteristics (for example, particle size distribution, organic carbon content, and total solids). These procedures can also be used to conduct bioconcentration tests and in situ tests, and to assess the toxicity of potentially contaminated field sediments, or of materials such as sewage sludge, oils, particulate matter, and solutions of toxicants added to sediments. A median lethal concentration (LC50) or median sublethal effect concentration (EC50) of toxicants or of highly contaminated sediment mixed into uncontaminated sediment can be determined. Materials adhering to sediment particles or dissolved in interstitial water can be tested.

1.5The results of 10-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality relative to a control or reference sediment. The results of 20 to 28-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality or growth relative to a control or reference sediment.

1.6This guide is arranged as follows:

Section

Referenced Documents

2

Terminology

3

Summary of Guide

4

Significance and Use

5

Interferences

6

Apparatus

7

 Facilities

7.1

 Construction Materials

7.2

 Test Chambers

7.3

 Cleaning

7.4

 Acceptability

7.5

Hazards

8

Test Water

9

 General Requirements

9.1

 Source

9.2

 Preparation

9.3

 Characterization

9.4

Test and Control Sediments

10

 General

10.1

 Characterization

10.2

 Control Sediment

10.3

 Field-Collected Test Sediment

10.4

 Reference Sediment

10.5

 Laboratory-Spiked Test Sediment

10.6

 Test Concentration(s)

10.7

 Addition of Toxicant to Sediment

10.8

Test Organisms

11

  Species

11.1

  Age

11.2

  Feeding

11.3

  Source

11.4

  Collection and Handling

11.5

  Quality

11.6

 Experimental Design

12

  Controls

12.2

  Field Survey Design

12.3

  Laboratory Experiments

12.4

 Procedure

13

  Dissolved Oxygen

13.1

  Temperature

13.2

  Salinity

13.3

  Light

13.4

  Feeding

13.5

  Beginning of Test

13.6

  Duration of Test

13.7

  Biological Data

13.8

  Other Measurements

13.9

 Analytical Methodology

14

 Acceptability of Test

15

 Interpretation of Results

16

 Report

17

 Keywords

18

 Annexes

  Neanthes arenaceodentata

Annex A1

  Neanthes virens

Annex A2


1.7The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.8This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8.

ASTM E 1391 : 2003 : R2014 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1367 : 2003 : R2014 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments
ASTM E 1850 : 2004 : R2019 Standard Guide for Selection of Resident Species as Test Organisms for Aquatic and Sediment Toxicity Tests
ASTM E 3163 : 2018 Standard Guide for Selection and Application of Analytical Methods and Procedures Used during Sediment Corrective Action
ASTM E 2591 : 2007 : R2013 Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians

ASTM E 1367 : 2003 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1023 : 1984 : R2007 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM E 1391 : 2002 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing
ASTM D 4447 : 2015 Standard Guide for Disposal of Laboratory Chemicals and Samples
ASTM E 1706 : 2005 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water
ASTM D 1129 : 2003 Standard Terminology Relating to Water
ASTM D 4447 : 1984 : R2003 Standard Guide for Disposal of Laboratory Chemicals and Samples
ASTM E 1525 : 1994 Standard Guide for Designing Biological Tests with Sediments
ASTM D 1129 : 2013 Standard Terminology Relating to Water
ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
ASTM E 1391 : 2003 : R2014 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM D 3976 : 1992 : R2005 Standard Practice for Preparation of Sediment Samples for Chemical Analysis
ASTM D 4447 : 2015 : REDLINE Standard Guide for Disposal of Laboratory Chemicals and Samples
ASTM D 4447 : 2006 Standard Guide for Disposal of Laboratory Chemicals and Samples
ASTM E 1525 : 1994 : REV A Standard Guide for Designing Biological Tests with Sediments
ASTM E 1023 : 1984 : R2014 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM D 3976 : 1992 : R2001 Standard Practice for Preparation of Sediment Samples for Chemical Analysis
ASTM E 1367 : 2003 : R2014 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM D 1129 : 2006 Standard Terminology Relating to Water
ASTM E 1023 : 1984 : R2002 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM D 3976 : 1992 : R2010 Standard Practice for Preparation of Sediment Samples for Chemical Analysis
ASTM E 1706 : 2000 : EDT 1 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates
ASTM E 1706 : 2020 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM D 3976 : 1992 : R1996 Standard Practice for Preparation of Sediment Samples for Chemical Analysis
ASTM E 1391 : 1994 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing
ASTM E 1391 : 2003 : R2008 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1706 : 2000 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Fresh Water Invertebrates
ASTM E 1706 : 2005 : EDT 1 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1192 : 1997 : R2008 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
IEEE/ASTM SI_10-2010 American National Standard for Metric Practice
ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
ASTM D 1129 : 2002 Standard Terminology Relating to Water
ASTM E 1367 : 2003 : R2008 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1023 : 1984 : R1996 Standard Guide for Assessing the Hazard of a Material to Aquatic Organisms and Their Uses
ASTM D 3976 : 1992 PRACTICE FOR PREPARATION OF SEDIMENT SAMPLES FOR CHEMICAL ANALYSIS
ASTM D 4447 : 2021 Standard Guide for Disposal of Laboratory Chemicals and Samples
ASTM E 1367 : 1992 Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods
ASTM E 1391 : 2003 Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates
ASTM E 1192 : 1997 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM D 1129 : 2004 Standard Terminology Relating to Water
ASTM D 3976 : 1992 : R2015 Standard Practice for Preparation of Sediment Samples for Chemical Analysis
ASTM E 1367 : 2003 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Estuarine and Marine Invertebrates
ASTM E 1706 : 2005 : R2010 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates (Withdrawn 2019)
ASTM E 1525 : 2002 : R2008 Standard Guide for Designing Biological Tests with Sediments
ASTM D 4447 : 1984 : R1997 Standard Guide for Disposal of Laboratory Chemicals and Samples
ASTM E 1706 : 2000 : EDT 2 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1192 : 1997 : R2003 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1706 : 2004 Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM E 1241 : 2005 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
ASTM D 4447 : 2010 Standard Guide for Disposal of Laboratory Chemicals and Samples
ASTM E 729 : 1996 Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1192 : 1997 : R2014 Standard Guide for Conducting Acute Toxicity Tests on Aqueous Ambient Samples and Effluents with Fishes, Macroinvertebrates, and Amphibians
ASTM E 1706 : 2019 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
ASTM D 1129 : 2010 Standard Terminology Relating to Water
ASTM E 1525 : 2002 Standard Guide for Designing Biological Tests with Sediments
ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
ASTM E 1241 : 2005 : R2013 Standard Guide for<brk type="line"/> Conducting Early Life-Stage Toxicity Tests with Fishes (Withdrawn 2022)
ASTM D 1129 : 2001 Standard Terminology Relating to Water
ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
ASTM E 1241 : 1998 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes
ASTM E 1367 : 1999 Standard Guide for Conducting 10-day Static Sediment Toxicity Tests with Marine and Estuarine Amphipods
ASTM E 1525 : 2002 : R2014 Standard Guide for Designing Biological Tests with Sediments

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