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ASTM E 1873 : 2006

Withdrawn

Withdrawn

A Withdrawn Standard is one, which is removed from sale, and its unique number can no longer be used. The Standard can be withdrawn and not replaced, or it can be withdrawn and replaced by a Standard with a different number.

Standard Guide for Detection of Nucleic Acid Sequences by the Polymerase Chain Reaction Technique (Withdrawn 2014)

Available format(s)

Hardcopy , PDF

Withdrawn date

05-08-2014

Language(s)

English

Published date

01-11-2006

€67.30
Excluding VAT

Committee
E 55
DocumentType
Guide
Pages
9
PublisherName
American Society for Testing and Materials
Status
Withdrawn
Supersedes

1.1 This guide covers guidelines, recommendations, basic considerations, criteria, and principles to be employed when developing, utilizing, or assessing PCR procedures and specific protocols for the amplification and detection of nucleic acid sequences. This guide is not intended to be a standard procedure with a list of requirements for PCR detection of nucleic acids. This guide is intended to provide information that will assist the user in obtaining quality and reliable data.

1.2 Nucleic acid targets for PCR include DNA, as well as RNA ; RNA sequences are suitable targets for PCR following reverse transcription of the RNA to complementary DNA (cDNA). This type of amplification technique is known as reverse transcription-PCR (RT-PCR).

1.3 This guide has been developed for use in any molecular biology/biotechnology laboratory. This includes, but is not limited to, laboratories that specialize in the diagnosis of human, animal, plant, or bacterial diseases.

1.4 This guide conveys the general procedural terminology of PCR technology used for the detection of nucleic acids.

1.5 This guide is general; it does not cover the additional guidance that would be needed for specific applications, for example, for the PCR detection of nucleic acid sequences of specific microorganisms.

1.6 This guide does not cover details of the various methods that can be utilized to identify PCR-amplified DNA sequences.

1.7 This guide does not cover specific variations of the basic PCR or RT-PCR technology (for example, quantitative PCR, real-time PCR, multiplex PCR, and in situ PCR), and it does not cover details of instrument calibration.

1.8 Warning-Laboratory work involving certain clinical specimens and microorganisms can be hazardous to personnel. Warning-Biosafety level 2 (or higher) facilities are recommended for biohazard work (). Safety guidelines should be adhered to in accordance with CLSI M29-A2 and other recommendations ().

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