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ASTM E 2563 : 2013

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Standard Practice for Enumeration of Non-Tuberculosis Mycobacteria in Aqueous Metalworking Fluids by Plate Count Method

Available format(s)

Hardcopy , PDF

Superseded date

13-10-2023

Superseded by

ASTM E 2563 : 2018

Language(s)

English

Published date

01-07-2013

€59.22
Excluding VAT

Committee
E 34
DocumentType
Standard Practice
Pages
3
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

1.1This practice covers the detection and enumeration of viable and culturable rapidly growing Mycobacteria (RGM), or non-tuberculosis Mycobacteria (NTM) in aqueous metalworking fluids (MWF) in the presence of high non-mycobacterial background population using standard microbiological culture methods.

1.2The detection limit is one colony forming unit (CFU)/mL metalworking fluid.

1.3This practice involves culture of organisms classified as Level 2 pathogens, and should be undertaken by a trained microbiologist in an appropriately equipped facility. The microbiologist should also be capable of distinguishing the diverse colonies of Mycobacteria from other microorganism colonies on a Petri dish and capable of confirming Mycobacteria by acid fast staining method

1.4This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM E 2275 : 2014 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
ASTM E 2148 : 2016 Standard Guide for Using Documents Related to Metalworking or Metal Removal Fluid Health and Safety
ASTM E 2889 : 2012 : R2017 Standard Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment
ASTM D 5465 : 2016 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

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