ASTM E 2647 : 2013
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown Using Drip Flow Biofilm Reactor with Low Shear and Continuous Flow
Hardcopy , PDF
06-06-2020
English
01-04-2013
Committee |
E 35
|
DocumentType |
Test Method
|
Pages |
6
|
PublisherName |
American Society for Testing and Materials
|
Status |
Superseded
|
SupersededBy | |
Supersedes |
1.1This test method specifies the operational parameters required to grow a repeatable2 Pseudomonas aeruginosa biofilm close to the air/liquid interface in a reactor with a continuous flow of nutrients under low fluid shear conditions. The resulting biofilm is representative of generalized situations where biofilm exists at the air/liquid interface under low fluid shear rather than representative of one particular environment.
1.2This test method uses the drip flow reactor. The drip flow reactor (DFR) is a plug flow reactor with laminar flow resulting in low fluid shear. The reactor is versatile and may also be used for growing and/or characterizing biofilms of different species, although this will require changing the operational parameters to optimize the method based upon the growth requirements of the new organism.
1.3This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log colony forming units per surface area.
ASTM D 5465 : 1993 : R2012 | Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods |
ASTM D 5465 : 1993 : R2004 | Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods |
ASTM D 5465 : 2016 | Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods |
ASTM D 5465 : 1993 : R1998 | Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods |
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