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ASTM E 2647 : 2013

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Standard Test Method for Quantification of Pseudomonas aeruginosa Biofilm Grown Using Drip Flow Biofilm Reactor with Low Shear and Continuous Flow

Available format(s)

Hardcopy , PDF

Superseded date

06-06-2020

Superseded by

ASTM E 2647 : 2020

Language(s)

English

Published date

01-04-2013

€67.30
Excluding VAT

Committee
E 35
DocumentType
Test Method
Pages
6
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy
Supersedes

1.1This test method specifies the operational parameters required to grow a repeatable2 Pseudomonas aeruginosa biofilm close to the air/liquid interface in a reactor with a continuous flow of nutrients under low fluid shear conditions. The resulting biofilm is representative of generalized situations where biofilm exists at the air/liquid interface under low fluid shear rather than representative of one particular environment.

1.2This test method uses the drip flow reactor. The drip flow reactor (DFR) is a plug flow reactor with laminar flow resulting in low fluid shear. The reactor is versatile and may also be used for growing and/or characterizing biofilms of different species, although this will require changing the operational parameters to optimize the method based upon the growth requirements of the new organism.

1.3This test method describes how to sample and analyze biofilm for viable cells. Biofilm population density is recorded as log colony forming units per surface area.

1.4Basic microbiology training is required to perform this test method.

1.5The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.6This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM D 5465 : 1993 : R2012 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM D 5465 : 1993 : R2004 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM D 5465 : 2016 Standard Practices for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods
ASTM D 5465 : 1993 : R1998 Standard Practice for Determining Microbial Colony Counts from Waters Analyzed by Plating Methods

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