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ASTM E 2694 : 2016 : REDLINE

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

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Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

Available format(s)

PDF

Superseded date

05-01-2022

Language(s)

English

Published date

01-10-2016

€74.48
Excluding VAT

CONTAINED IN VOL. 11.03, 2017 Gives a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

Committee
E 34
DocumentType
Redline
Pages
14
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

1.1This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

1.2The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard and computation to pg ATP/mL.

1.3This test method is equally suitable for use in the laboratory or field.

1.4The test method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400 000 pg ATP/mL.

1.5Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi.

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

ASTM D 7687 : 2017 : REDLINE Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
ASTM E 2148 : 2016 : REDLINE Standard Guide for Using Documents Related to Metalworking or Metal Removal Fluid Health and Safety
ASTM E 2275 : 2014 : REDLINE Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
ASTM E 2889 : 2012 : R2017 Standard Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment
ASTM D 4012 : 2015 : REDLINE Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM E 2889 : 2012 Standard Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment
ASTM E 2275 : 2019 : REDLINE Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance

ASTM E 1326 : 2015-07 GUIDE FOR EVALUATING NON-CULTURE MICROBIOLOGICAL TESTS
ASTM D 4840 : 1999 Standard Guide for Sampling Chain-of-Custody Procedures
ASTM D 1129 : 2013 : REDLINE Standard Terminology Relating to Water
ASTM E 177 : 2014 : REDLINE Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
ASTM E 1497 : 2017 : REDLINE Standard Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids
CFR 29(PT1910.1000 TO END) : 0 LABOR - OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR
ASTM D 4012 : 2015 : REDLINE Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water

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