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ASTM E 2694 : 2021

Current

Current

The latest, up-to-date edition.

Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

10-11-2021

€61.92
Excluding VAT

Committee
E 34
DocumentType
Test Method
Pages
14
PublisherName
American Society for Testing and Materials
Status
Current
Supersedes

1.1This test method provides a protocol for capturing, extracting, and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWFs).

1.2The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLUs) which are converted by comparison with an ATP standard and computation to pg ATP/mL.

1.3This test method is equally suitable for use in the laboratory or field.

1.4The test method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400 000 pg ATP/mL.

1.5Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi.

1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.8This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM D 7687 : 2021 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM E 2275 : 2019 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
ASTM E 2523 : 2013 : R2018 Standard Terminology for Metalworking Fluids and Operations
ASTM E 979 : 2020 Standard Practice for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
ASTM E 2889 : 2012 : R2017 Standard Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment
ASTM E 2148 : 2021 Standard Guide for Using Documents Related to Metalworking or Metal Removal Fluid Health and Safety

ASTM E 1497 : 2023 Standard Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids
ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
ASTM D 4012 : 2023 : REV A Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM D 1129 : 2013 : R2020 : EDT 2 Standard Terminology Relating to Water
ASTM D 1129 : 2013 : R2020 : EDT 1 Standard Terminology Relating to Water
ASTM E 691 : 2023 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E 2523 : 2023 Standard Terminology for Metalworking Fluids and Operations
ASTM E 691 : 2022 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM D 4012 : 2023 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
ASTM E 691 : 2021 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
ASTM E 1497 : 2017 Standard Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids

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