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ASTM E 3161 : 2018

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by

Standard Practice for Preparing a Pseudomonas aeruginosa or Staphylococcus aureus Biofilm using the CDC Biofilm Reactor

Available format(s)

Hardcopy , PDF

Superseded date

28-01-2022

Superseded by

ASTM E 3161 : 2021

Language(s)

English

Published date

29-06-2018

€67.30
Excluding VAT

1.1This practice specifies the parameters for growing aPseudomonas aeruginosa(ATCC 15442) orStaphylococcus aureus(ATCC 6538) biofilm that can be used for disinfectant efficacy testing using the Test Method for Evaluating Disinfectant Efficacy AgainstPseudomonas aeruginosaBiofilm Grown in CDC Biofilm Reactor Using Single Tube Method (E2871) or in an alternate method capable of accommodating the coupons used in the CDC Biofilm Reactor. The resulting biofilm is representative of generalized situations where biofilm exist on hard, non-porous surfaces under shear rather than being representative of one particular environment. Additional bacteria may be grown using the basic procedure outlined in this document, however, alternative preparation procedures for frozen stock cultures and biofilm generation (for example, medium concentrations, baffle speed, temperature, incubation times, coupon types, etc.) may be necessary.1.2This practice uses the CDC Biofilm Reactor created by the Centers for Disease Control and Prevention(1).2The CDC Biofilm Reactor is a continuously stirred tank reactor (CSTR) with high wall shear. The reactor is versatile and may also be used for growing or characterizing various species of biofilm, or both(2-4)provided appropriate adjustments are made to the growth media and operational parameters of the reactor.1.3Basic microbiology training is required to perform this practice.1.4Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this practice.1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.1.6This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Committee
E 35
DocumentType
Standard Practice
Pages
8
PublisherName
American Society for Testing and Materials
Status
Superseded
SupersededBy

1.1This practice specifies the parameters for growing a Pseudomonas aeruginosa (ATCC 15442) or Staphylococcus aureus (ATCC 6538) biofilm that can be used for disinfectant efficacy testing using the Test Method for Evaluating Disinfectant Efficacy Against Pseudomonas aeruginosa Biofilm Grown in CDC Biofilm Reactor Using Single Tube Method (E2871) or in an alternate method capable of accommodating the coupons used in the CDC Biofilm Reactor. The resulting biofilm is representative of generalized situations where biofilm exist on hard, non-porous surfaces under shear rather than being representative of one particular environment. Additional bacteria may be grown using the basic procedure outlined in this document, however, alternative preparation procedures for frozen stock cultures and biofilm generation (for example, medium concentrations, baffle speed, temperature, incubation times, coupon types, etc.) may be necessary.

1.2This practice uses the CDC Biofilm Reactor created by the Centers for Disease Control and Prevention (1).2 The CDC Biofilm Reactor is a continuously stirred tank reactor (CSTR) with high wall shear. The reactor is versatile and may also be used for growing or characterizing various species of biofilm, or both (2-4) provided appropriate adjustments are made to the growth media and operational parameters of the reactor.

1.3Basic microbiology training is required to perform this practice.

1.4Units—The values stated in SI units are to be regarded as standard. No other units of measurement are included in this practice.

1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.6This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

ASTM E 2871 : 2019 Standard Test Method for Determining Disinfectant Efficacy Against Biofilm Grown in the CDC Biofilm Reactor Using the Single Tube Method

ASTM E 2756 : 2019 Standard Terminology Relating to Antimicrobial and Antiviral Agents
ASTM E 2756 : 2010 Standard Terminology Relating to Antimicrobial and Antiviral Agents
ASTM E 2871 : 2013 Standard Test Method for Evaluating Disinfectant Efficacy Against <i>Pseudomonas aeruginosa</i> Biofilm Grown in CDC Biofilm Reactor Using Single Tube Method
ASTM E 2756 : 2018 Standard Terminology Relating to Antimicrobial and Antiviral Agents
ASTM E 2871 : 2012 Standard Test Method for Evaluating Disinfectant Efficacy against <emph type="bdit">Pseudomonas aeruginosa</emph> Biofilm Grown in CDC Biofilm Reactor using Single Tube Method
ASTM E 2871 : 2019 Standard Test Method for Determining Disinfectant Efficacy Against Biofilm Grown in the CDC Biofilm Reactor Using the Single Tube Method

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