ASTM F 3088 : 2014
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions
Hardcopy , PDF
17-06-2022
English
08-07-2014
Important note : Users cannot be edited or removed once added to your Multi user PDF.CONTAINED IN VOL. 13.02, 2014 Defines a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments.
| Committee |
F 04
|
| DocumentType |
Test Method
|
| Pages |
7
|
| PublisherName |
American Society for Testing and Materials
|
| Status |
Superseded
|
| SupersededBy |
1.1This test method covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. This approach measures the force required to detach cells from a substrate. Adhesion, among many variables, may vary due to changes in the phenotype of the cells.
1.2This test method does not cover methods to verify the uniformity of coating of surfaces, nor does it cover methods for characterizing surfaces.
1.3The cells may include adult, progenitor or stem cells from any species. The types of cells may include chondrocytes, fibroblasts, osteoblast, islet cells, or other relevant adherent cell types.
1.4This test method does not cover methods for isolating or harvesting of cells. This test method does not cover test methods to quantitate changes in gene expression, or changes in biomarker type or concentration, as identified by immunostaining. Nor does this test method cover quantitative image analysis techniques.
1.5This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
| ASTM F 2664 : 2011 : REDLINE | Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods |
| ASTM F 2739 : 2019 | Standard Guide for Quantifying Cell Viability and Related Attributes within Biomaterial Scaffolds |
| ASTM F 2944 : 2012 | Standard Test Method for Automated Colony Forming Unit (CFU) Assays—Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture |
| ASTM F 2739 : 2008 | Standard Guide for Quantitating Cell Viability Within Biomaterial Scaffolds |
| ASTM F 2664 : 2011 | Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods |
| ASTM F 2603 : 2006 | Standard Guide for Interpreting Images of Polymeric Tissue Scaffolds |
| ASTM F 2664 : 2019 : EDT 1 | Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods |
| ASTM F 2739 : 2016 | Standard Guide for Quantifying Cell Viability within Biomaterial Scaffolds |
| ASTM F 2664 : 2019 | Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods |
| ASTM F 2664 : 2007 | Standard Guide for Assessing the Attachment of Cells to Biomaterial Surfaces by Physical Methods |
| ASTM F 2603 : 2006 : R2012 | Standard Guide for Interpreting Images of Polymeric Tissue Scaffolds |
| ASTM F 2944 : 2020 | Standard Practice for Automated Colony Forming Unit (CFU) Assays—Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture |
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