BS EN 16418:2014
Current
The latest, up-to-date edition.
Paper and board. Determination of the cytotoxicity of aqueous extracts using a metabolically competent hepatoma cell line (HepG2)
Hardcopy , PDF
English
30-04-2014
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Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Cell line
7 Food simulants used for testing
8 Cleaning laboratory glassware
9 Equipment
10 Preparation of specimens
11 Cytotoxicity assessment
12 Expression of the results
13 Interpretation of the results
14 Precision
15 Test report
Annex A (informative) - 96-well plates configuration
Annex B (informative) - RNA Synthesis rate
inhibition cytotoxicity test work flow
Annex C (informative) - Validation of the two
methods (option A and B)
Bibliography
Defines a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG[2] cell line.
| Committee |
PAI/11
|
| DevelopmentNote |
Supersedes 12/30259424 DC. (04/2014)
|
| DocumentType |
Standard
|
| Pages |
24
|
| PublisherName |
British Standards Institution
|
| Status |
Current
|
| Supersedes |
This European Standard specifies a test method for the laboratory assessment of the potential cytotoxic effect of paper and board intended to come into contact with foodstuffs using specifically the HepG2 cell line.
Compared to the EN 15845[1], HepG2 cells are more representative of a human oral exposure to xenobiotics, due to the presence in the cells of phase I, II and III enzymes of the metabolism.
| Standards | Relationship |
| EN 16418:2014 | Identical |
| EN 645:1993 | Paper and board intended to come into contact with foodstuffs - Preparation of a cold water extract |
| EN 15845:2010 | Paper and board - Determination of the cytotoxicity of aqueous extracts |
| ISO 3696:1987 | Water for analytical laboratory use — Specification and test methods |
| EN ISO 3696:1995 | Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) |
| EN 647:1993 | Paper and board intended to come into contact with foodstuffs - Preparation of a hot water extract |
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