BS EN ISO 15216-1:2017+A1:2021
Current
The latest, up-to-date edition.
Microbiology of the food chain. Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR Method for quantification
Hardcopy , PDF
English
30-04-2021
Important note : Users cannot be edited or removed once added to your Multi user PDF.| Committee |
AW/9
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| DocumentType |
Standard
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| Pages |
54
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| PublisherName |
British Standards Institution
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| Status |
Current
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| Supersedes |
This document specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.
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