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BS EN ISO 20418-1:2018

Current

Current

The latest, up-to-date edition.

Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres Peptide detection using LC-ESI-MS with protein reduction

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

05-06-2018

€165.94
Excluding VAT

Committee
TCI/100
DocumentType
Standard
ISBN
9780580897023
Pages
20
PublisherName
British Standards Institution
Status
Current

This document specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC).

This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e.g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated.

This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO1833series and/or the ISO17751series.

This document does not apply if fibres of the same animal species are present (e.g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e.g. ISO17751series).

Standards Relationship
ISO 20418-1:2018 Identical
EN ISO 20418-1:2018 Identical

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