BS EN ISO 22959:2009

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents, materials and standards
6 Apparatus
7 Sampling and preparation of the test sample
8 Sample preparation
9 Procedure
   9.1 HPLC analysis
   9.2 Identification of PAHs
10 Calculation of individual PAHs
11 Method validation data
   11.1 Accuracy
   11.2 Within-laboratory precision
   11.3 Recovery
   11.4 Dynamic range
   11.5 Limit of quantification
12 Precision
   12.1 International collaborative trial
   12.2 Repeatability
   12.3 Reproducibility
13 Test report
Annex A (informative) - Example of the individual parts
         of an HPLC system
Annex B (informative) - Example of the operating conditions
        of the pumps of the HPLC system
Annex C (informative) - Example of the operating conditions
        of the column thermostat and the detector of the
        HPLC system
Annex D (informative) - Example of the autosampler programme
        of the HPLC system
Annex E (informative) - Tubing connections of the HPLC system
Annex F (informative) - Chromatogram of a standard calibration
        sample
Annex G (informative) - Determination precision for a sunflower
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex H (informative) - Determination precision for an olive
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex I (informative) - Determination precision for a soybean
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex J (informative) - Determination precision for a coconut
        oil, range 0,1 [mu]g/kg to 3,5 [mu]g/kg
Annex K (informative) - Interlaboratory test
Bibliography

Describes a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.

This International Standard specifies a high performance liquid chromatographic (HPLC) procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible fats and oils.

The method has been validated for coconut (CN), olive (OV), sunflower (SF), and soybean (BO) oil, and is possibly applicable to other oils, dependent on the determination of appropriate parameters.

The lowest level of quantification for the PAHs is 0,1µg/kg. The lowest possible amount of each PAH which can be distinguished from the baseline noise has not been determined. The validated concentration range of the method is 0,1µg/kg to 3,5µg/kg for each individual PAH. For samples containing (light) PAH contents>3,5µg/kg, dilution to bring the contents into the validated range is possible. It is also possible to adjust the range of the calibration curves. However, ranges exceeding 3,5µg/kg have not been validated.

PAHs which can be determined by this method are: anthracene, phenanthrene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo[e]pyrene, benzo[a]pyrene, perylene, benzo[ghi]perylene, anthanthrene, dibenzo[a,h]anthracene, coronene, indeno[1,2,3-cd]pyrene, benzo[a]fluoranthene, benzo[b]fluoranthene, benzo[k]fluoranthene.