BS ISO 11350:2012

Foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Interferences
5 Principle
6 Apparatus and materials
7 Reagents, media and dilutions
8 Sampling and samples
9 Procedure
10 Validity criteria
11 Assessment criteria
12 Test report
Annex A (normative) - Nutrient broth and agar
Annex B (normative) - Preparation of ampicillin agar
        plates and stock cultures
Annex C (normative) - Checking of genotype
Annex D (normative) - S9 fraction
Annex E (informative) - Example for application of
        samples on a 24 well plate
Annex F (informative) - Example for reporting
Annex G (informative) - Testing of chemicals
Annex H (informative) - Precision data
Annex I (informative) - Statistical assessment
Annex J (informative) - Measurement of the lowest
        ineffective dilution (LID) of a waste water - A
        simplified evaluation for testing of waste water
Annex K (informative) - Use of additional tester strains
Bibliography

Describes a method for the determination of the genotoxic potential of water and waste water using the bacterial strains Salmonella enterica subsp. enterica serotype Typhimurium TA 98 and TA 100 in a fluctuation assay.

This International Standard specifies a method for the determination of the genotoxic potential of water and waste water using the bacterial strains Salmonella enterica subsp. enterica serotype Typhimurium TA 98 and TA 100 in a fluctuation assay. This combination of strains is able to measure the genotoxicity of chemicals that induce point mutations (base pair substitutions and frameshift mutations) in genes coding for enzymes that are involved in the biosynthesis of the amino acid, histidine.

NOTE1 ISO13829^[8] applies for the measurement of genotoxicity of samples containing DNA-crosslinking agents.

This method is applicable to:

• fresh water;

• waste water;

• aqueous extracts and leachates;

• eluates of sediments (fresh water);

• pore water;

• aqueous solutions of single substances or of chemical mixtures;

• drinking water.

NOTE2 When testing drinking water, extraction and pre-concentration of water samples can prove necessary.